APOE4 is the strongest genetic risk factor associated with late-onset Alzheimer’s disease (AD). To address the underlying mechanism, we develop cerebral organoid models using induced pluripotent stem cells (iPSCs) with APOE ε3/ε3 or ε4/ε4 genotype f
com 【 Abstract】 The apolipoprotein E ( ApoE) is involved in cholesterol and lipid metabolism in the brain, transporting cholesterol to neurons and removing lipid debris to facilitate myelin repair. ApoE4, one of three subtypes of ApoE, is the most important genetic risk factor for sporadic ...
com 【 Abstract】 The apolipoprotein E ( ApoE) is involved in cholesterol and lipid metabolism in the brain, transporting cholesterol to neurons and removing lipid debris to facilitate myelin repair. ApoE4, one of three subtypes of ApoE, is the most important genetic risk factor for sporadic ...
In response, astrocytes (FIG. 1, Step 2) and macrophages synthesize and release ApoE within the lesion to scavenge cholesterol from both cellular and myelin debris. Using the activity of the 3,3-hydroxy-methylglutaryl-CoA reductase (HMG-COA reductase) to monitor cholesterol synthesis, it was ...
Cell media were harvested and centrifuged at 800×gfor 3 min to remove cell debris. Immunoprecipitation using cell media was performed using an anti-HA antibody in the presence of protein-G Sepharose (GE Healthcare, Chicago, IL, USA) for 18 h at 4 °C. The beads were then washed three...