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Single-nucleus RNA sequencing analysis revealed striking gene expression changes in all cell types, with astrocytic APOE4 removal decreasing disease-associated gene signatures in neurons, oligodendrocytes, astrocytes, and microglia. Removal of astrocytic APOE4 decreased tau-induced synaptic loss and ...
The ε4 allele of the apolipoprotein E gene (APOE4) is a strong genetic risk factor for Alzheimer’s disease (AD) and multiple vascular conditions. ApoE is abundantly expressed in multiple brain cell types, including astrocytes, microglia, and vascular mural cells (VMCs). Here, we show that...
After incubation, we depleted myelin debris by 30% isotonic Percoll (P1644; Sigma-Aldrich) gradient centrifugation and obtained mononuclear cell suspensions in DMEM/F12 medium with ice-cold 10% heat-inactivated fetal bovine serum. We prepared unstained controls from mixtures of different sample cell ...
com 【 Abstract】 The apolipoprotein E ( ApoE) is involved in cholesterol and lipid metabolism in the brain, transporting cholesterol to neurons and removing lipid debris to facilitate myelin repair. ApoE4, one of three subtypes of ApoE, is the most important genetic risk factor for sporadic ...
com 【 Abstract】 The apolipoprotein E ( ApoE) is involved in cholesterol and lipid metabolism in the brain, transporting cholesterol to neurons and removing lipid debris to facilitate myelin repair. ApoE4, one of three subtypes of ApoE, is the most important genetic risk factor for sporadic ...
(LipidSpot) showed greater LD accumulation inAPOE4/4iMG compared with isogenicAPOE3/3iMG, similar to recent reports in isogenic iMG4and astrocytes20. However, treatment of iMG with fibrillar Aβ (fAβ) led to a strong increase in LD accumulation which was exacerbated by the presence of the...
We also use optional cookies for advertising, personalisation of content, usage analysis, and social media. By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with...
After incubation, we depleted myelin debris by 30% isotonic Percoll (P1644; Sigma-Aldrich) gradient centrifugation and obtained mononuclear cell suspensions in DMEM/F12 medium with ice-cold 10% heat-inactivated fetal bovine serum. We prepared unstained controls from mixtures of different sample cell ...
In response, astrocytes (FIG. 1, Step 2) and macrophages synthesize and release ApoE within the lesion to scavenge cholesterol from both cellular and myelin debris. Using the activity of the 3,3-hydroxy-methylglutaryl-CoA reductase (HMG-COA reductase) to monitor cholesterol synthesis, it was ...