18,19,20 The native activity of Acrs to inhibit CRISPR-Cas nuclease activity has been leveraged to increase gene editing precision through the addition of AcrIIA4, an Acr that inhibits SpCas9 activity by mimicking double-stranded DNA (dsDNA), after SpCas9 ribonucleoprotein (RNP) delivery.19,21...
Remarkably, dsRNA, but not other nucleic acids (ssRNA, ssDNA, dsDNA), efficiently promoted the fusion of TRIM25 and G3BP1 droplets (Fig. 2h, i). Thus, dsRNA specifically promotes TRIM25-G3BP1 co-condensation. Next, we asked whether TRIM25 could alter the LLPS property of G3BP1. We ...
Also, in the THP-1 cell line there was a 2-fold increase in the level of dsDNA breaks against CLA (Fig. 6). We showed here that increase in the level of γH2AX, induced by cladribine and by drug’s derivatives, were significantly reduced by the ATR inhibitor VE-821. Figure 6 The ...
The quantity of genomic DNA was measured by qubit dsDNA BR assay kit (Invitrogen, USA), and the quality was verified on 1% agarose gel. 2.7.2. 16S rRNA gene sequence and data analysis The V4 region of 16S rRNA gene was amplified from the DNA extractions with degenerate PCR primers 515...
Qubit dsDNA High Sensitivity Assay kit Life Technologies Cat# Q32851 Bioanalyser High Sensitivity DNA kit Agilent Cat# 5067-4626 NEBNext® Library Quant Kit for Illumina New England Biolabs Cat# E7630S/L Contact for Reagent and Resource Sharing Further information and requests for reagents may be...
Then, cDNA was used as a template for dsDNA sequences of genes of interest, and a final step aimed at partial sequencing of our genes. A RecA partial sequence showed about 1400 bps, cna was estimated with 1200 pbs, while fnbA was partially estimated at 1K bps (Figure 16). Gene ...
Background/Objectives: Due to the high frequency and severity of upper respiratory bacterial infections, probiotics could offer a new medical approach. We explored the antibacterial and anti-inflammatory properties of the new strain Lactiplantibacillus p
(TFA). Next, the peptides from the supernatant were removed and collected in a clean LoBind tube and the extracts were concentrated on speedvac. Finally, these digested proteins were resuspended in 0.1% formic acid and subjected to LC-MS/MS (Thermo Scientific, Waltham, MA, USA). The data...
A 0.2 mL aliquot of ONPG substrate (4 mg/mL) was then added to each test tube. When yellow color developed, the reaction was stopped by adding 0.5 mL of 1 M Na2CO3 followed by vortexing. Reaction mixtures were centrifuged (15k rpm, 3 min), and the absorbance values of the supernatants...
2.2. DNA Preparation, Metagenomic Sequencing, and Taxonomic Annotation Genomic DNA was extracted using the QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) combined with the bead-beating method, as per the manufacturer's recommen- dations. DNA quantity was determined using the BR dsDNA Qubit...