To coat dsDNA in microtiter wells, tow different kinds of reagents including methylated 鈥揃SA and poly-l-lysine (for pre-coating) are used. Sera from systemic lupus erythematosus patients and from normal blood donors are used to assess sensitivity and specificity of our ELISA test in compared ...
In a normal range study with serum samples from healthy blood donors the following ranges have been established with the Anti-dsDNA tests: Anti-dsDNA IgG [IU/ml] Cut-Off: 20 Positive results should be verified concerning the entire clinical status of the ...
dsDNA and anti-Smith (Sm) antibodies are relatively specific for SLE: 95–97%, and 50–99%, respectively. While anti-dsDNA antibodies seem to correlate with thedisease activity, anti-Sm antibodies tend to persist after normalization of anti-dsDNA titers. An interstitiallung disease(ILD) ...
Of all anti-dsDNA antibody detection methods, the Crithidia luciliae immunofluorescence test (CLIF) is considered to have the highest specificity for systemic lupus erythematosus (SLE).The objective of this report is to evaluate whether the presence of anti-dsDNA antibodies detected by the CLIF metho...
quantification is expressed in μg of DNA deposited (left y-axis, blue triangles), the signal from the anti-dsDNA antibodies is expressed as arbitrary units (a.u., right y-axis, green triangles). The difference between the two groups is non-significant, as calculated by the pairedt-test....
(Biochemistry) any of various proteins produced in the blood in response to the presence of an antigen. By becoming attached to antigens on infectious organisms antibodies can render them harmless or cause them to be destroyed. See alsoimmunoglobulin ...
Laboratory tests included complete blood count, serum creatinine, urine analysis, creatinine clearance and 24-h urine protein. Anti-dsDNA antibodies were assessed by indirect immunofluorescence, and C3 and C4 were quantified by nephelometry. Characteristics of the cohort All SLE inactive patients that we...
Raw sequencing reads obtained from sequencing the VH-CH1 region of B cells in peripheral blood were processed using a custom pipeline. The pipeline included adapter trimming and quality filtering; unique molecular identifier (UMI) processing; V(D)J gene annotation; clustering; quality control; and ...
In time-dependent analysis, anti-NCS antibodies showed a better fit over time than anti-dsDNA antibodies and C3. Limitations: the inclusion of a limited number of patients, not taking into consideration the impact of medications used influencing disease activity might have limited test results. In...
The level of white blood cells and proteins in CSF was not significantly different in the NMOSD with or without anti-SSA/Ro antibody. Patients who were seropositive for anti-SSA/Ro antibody more likely had anti-ANA antibody, anti-SSB/La antibody, and anti-dsDNA antibody, the positive rates...