Anti-dsDNA levels and anti-ENA profiles were determined by enzyme linked immune assay (ELISA) and immune-blotting (IB), respectively. Results Sex distribution of ANA positive patients was determined as 756 (77%) females and 226 (23%) males. Fifty per cent of the cases were from ...
antigenic source in compared with IF test and commercial kits 80%, 88% and 100%, 98% respectively, but when E.coli DNA is used 73%, 69% and 85%, 79%, respectively.Conclusion: The genomic DNA from calf thymus is a potentially useful source of antigen for detection of anti-dsDNA by ...
The serum titer can be readily quantified, and both low-avidity and high-avidity anti-dsDNA antibodies can be detected. False-positive test results can be seen when impure DNA is used as a substrate. The qualitative properties of anti-dsDNA, including antibody avidity, complement fixing property...
There is a wide spectrum of laboratory techniques available for the determination of anti-dsDNA, which challenges the use and interpretation of anti-dsDNA test results. The objective of this study is to review the definition and properties of anti-dsDNA antibodies, analyze the analytical and clinica...
Anti-dsDNA IgG [IU/ml] Cut-Off: 20 Positive results should be verified concerning the entire clinical status of the patient. Also every decision for therapy should be taken individually. It is recommended that each laboratory establishes its own normal and pathological ...
We also used this assay to screen SLE patient and normal plasmas for levels of anti-dsDNA IgG. The results of this assay correlate very well with the Farr assay, and therefore this approach may be useful in the development of informative and specific assays for a variety of autoantibodies. ...
In silico identification of viral anti-CRISPR proteins (Acrs) has relied largely on the guilt-by-association method using known Acrs or anti-CRISPR associated proteins (Acas) as the bait. However, the low number and limited spread of the characterized ar
In time-dependent analysis, anti-NCS antibodies showed a better fit over time than anti-dsDNA antibodies and C3. Limitations: the inclusion of a limited number of patients, not taking into consideration the impact of medications used influencing disease activity might have limited test results. In...
Objectives. The anti-dsDNA antibodies are a marker for Systemic Lupus Erythematosus (SLE) and 70–98% of patients test positive. We evaluated the demographic, clinical, laboratory, and therapeutical f...
ELISA is a useful and widely usedlaboratory testto screen for anti-dsDNA Abs [13,18] because of its high sensitivity, quantitative results, and simplicity, as well as relatively low cost compared to other assays [19,20], while CLIFT provides higher clinical specificity than ELISA [10]. There...