We hypothesize that this might be explained by the presence of anti-nucleosome antibodies, which can be detected by dsDNA/nucleosome ELISA or ENA-immunoblot. Clinicians should be aware of lower sensitivity (higher chance of false negatives) in EliA when interpreting anti-dsDNA test results. ...
intensive and rapid, and it avoids the use of radioactive reagents. The serum titer can be readily quantified, and both low-avidity and high-avidity anti-dsDNA antibodies can be detected. False-positive test results can be seen when impure DNA is used as a substrate....
AcrIF24 displays a weak binding to non-sequence-specific dsDNA (dsDNANS), with a KD value of 3.461 μM. Data are presented as mean values ± SD; n = 3. Raw data for these curves are shown in Supplementary Fig. 3. c EMSA used to test the binding affinities of AcrIF...
Our results demonstrate that DUF4297 and HerA assemble into a supramolecular complex, forming a dual-enzyme defense system with promiscuous dsDNA nuclease and ATPase activities. DUF4297 alone exists as a tail-to-tail dimer with limited nuclease activity, while HerA alone forms a nonplanar split spi...
suggesting that SAM binding promotes BREX sites recognition or results in more stable BrxX:DNA complexes. To further study BrxX substrate preferences we performed EMSAs with BREX site-containing dsDNA fragments of different lengths. Decreasing dsDNA length to 20 or 30 bp resulted in weaker binding...
“false positive” test. However, disassociation of the DNA from the dsDNAab binding site reduced but did not normalize, subsequent MPO-ANCA levels to suggest that MPO-ANCA may indeed have been present. In addition, in our study, elevated dsDNAab was not associated with elevated MPO-ANCA ...
A large number of studies have focused on the association of autoantibodies with specific symptoms of SLE: for example, it is well known that anti‐dsDNA antibodies, as well as antibodies to the collagen‐like region of the complement component C1q (anti‐C1q), are associated with renal ...
Serum was also studied for the levels of total IgG and IgM as well as the levels of anti–double-stranded DNA (anti-dsDNA) antibodies, using a mouse anti-dsDNA ELISA kit (Alpha Diagnostic International) in accordance with the manufacturer's instructions. Detection of antibody-secreting cells ...
(SseV isolate 1), an unclassified dsDNA virus (SYV1) and six MAGs (JAKMAA010000023.1, JAKMAB010000038.1, JAEMPR010000070.1, JAEMPR010000099.1, JAEMPR010000147.1, and JAEMPR010000199.1). Additionally, we have gathered several ORFs as low probability ADGs which were not included in our total ...
In the presence of symptoms of SLE, a positive antinuclear antibody test, and an elevated anti-dsDNA titer, SLE is the first diagnostic option [36]. Once the diagnosis is established, lupus activity can be monitored using quantitative techniques. The quantitative results obtained must be reported...