False-positive test results can be seen when impure DNA is used as a substrate. The qualitative properties of anti-dsDNA, including antibody avidity, complement fixing property, and Ig class, may affect their pathogenicity. The various clinical tests for anti-dsDNA preferentially measure antibodies ...
In silico identification of viral anti-CRISPR proteins (Acrs) has relied largely on the guilt-by-association method using known Acrs or anti-CRISPR associated proteins (Acas) as the bait. However, the low number and limited spread of the characterized ar
In the presence of symptoms of SLE, a positive antinuclear antibody test, and an elevated anti-dsDNA titer, SLE is the first diagnostic option [36]. Once the diagnosis is established, lupus activity can be monitored using quantitative techniques. The quantitative results obtained must be reported...
Anti-dsDNA levels were determined by the Farr radioimmunoassay, Quantalite ELISA, and the AtheNA Multi-Lyte ANA-II Plus Test System (AMLII). All assays were performed according to manufacturers' instructions in Clinical Laboratory Improvement Amendments (CLIA) certified laboratories. The sensitivity ...
AcrIF24 displays a weak binding to non-sequence-specific dsDNA (dsDNANS), with a KD value of 3.461 μM. Data are presented as mean values ± SD; n = 3. Raw data for these curves are shown in Supplementary Fig. 3. c EMSA used to test the binding affinities of AcrIF...
A large number of studies have focused on the association of autoantibodies with specific symptoms of SLE: for example, it is well known that anti‐dsDNA antibodies, as well as antibodies to the collagen‐like region of the complement component C1q (anti‐C1q), are associated with renal ...
Our results demonstrate that DUF4297 and HerA assemble into a supramolecular complex, forming a dual-enzyme defense system with promiscuous dsDNA nuclease and ATPase activities. DUF4297 alone exists as a tail-to-tail dimer with limited nuclease activity, while HerA alone forms a nonplanar split spi...
Objectives. The anti-dsDNA antibodies are a marker for Systemic Lupus Erythematosus (SLE) and 70–98% of patients test positive. We evaluated the demographic, clinical, laboratory, and therapeutical f...
Plasmacytoid dendritic cells (pDCs) are multifaceted immune cells executing various innate immunological functions. Their first line of defence consists in type I interferons (I-IFN) production upon nucleic acids sensing through endosomal Toll-like receptor (TLR) 7- and 9-dependent signalling pathways....
Results CB0313.1 improves hyperglycemia and insulin resistance in lepdb/db Mice To test the effects of CB0313.1 on glucose homeostasis, we performed oral glucose (OGTT) and insulin (ITT) tolerance tests. db-CB0313.1 mice (n = 8) exhibited significantly lower fasting glucose (Fig. 1a) and...