anti-DNA antibodiesELISABackground: Anti-dsDNA antibodies frequently found in the sera Systemic Lupus Erythematosus patients, particularly in active disease stage. Nowadays exploit different eukaryotic and prokaryotic dsDNA as antigen source and different reagents as binder. The aim of this study to ...
The serum titer can be readily quantified, and both low-avidity and high-avidity anti-dsDNA antibodies can be detected. False-positive test results can be seen when impure DNA is used as a substrate. The qualitative properties of anti-dsDNA, including antibody avidity, complement fixing property...
regression were performed to determine associations among predicting variables [age, gender, immunofluorescence pattern, and anti-nuclear antibody (ANA) titer] and anti-extractable nuclear antigen (ENA) and -dsDNA antibodies (Abs) in 1089 patients with positive fluorescent ANA (FANA) test results. Sam...
High avidity anti-dsDNA IgG antibodies are believed to play an important role in the pathogenesis of the autoimmune disease systemic lupus erythematosus (SLE) and therefore attempts have been made to reduce the concentration of these antibodies in the bloodstream of SLE patients. Previously we reporte...
Objectives. The anti-dsDNA antibodies are a marker for Systemic Lupus Erythematosus (SLE) and 70–98% of patients test positive. We evaluated the demographic, clinical, laboratory, and therapeutical f...
These results established a simple model to demonstrate that anti‐DNA antibodies can cause dysregulation of cytokine network mimicking systemic lupus erythematosus in culture of normal PBMCs, and emphasize again the importance of maintaining anti‐DNA antibodies at low levels by treatment.K. Inoue...
These and other data have established a framework for understanding the pathogenic role of anti‐DNA antibodies, but studies on normal cells are limited. Here, we used the monoclonal anti‐dsDNA antibody 2C10, 2‐kbp dsDNA and healthy human peripheral blood mononuclear cells (PBMCs) to test ...
Results were not influenced by gender, age, or duration of illness. Presence of antihistone antibodies in dementias might reflect an alteration of membrane fluidity and integrity with leakage of nuclear immunogens or disturbances of immune functions, as frequently observed in dementia disorders....
Objectives To assess the frequency of anti-dsDNA, increasing of CIC, hypocomplementemie in SLE and APS (in primary and secondary) and their connection with anticardiolipin antibodies (aCL) and clinical manifestations of the disease. Methods We studied 365 patients in mean age 31,9±10,9 years...
Autoantibody levels in serum and in IC were compared with Mann-Whitney9s U test between patients with high and low disease activity at baseline and between patients with and without treatment response during the follow-up period.ResultsAntibodies against dsDNA, SSA/Ro60 and Sm/RNP were found in...