intensive and rapid, and it avoids the use of radioactive reagents. The serum titer can be readily quantified, and both low-avidity and high-avidity anti-dsDNA antibodies can be detected. False-positive test results can be seen when impure DNA is used as a substrate....
These and other data have established a framework for understanding the pathogenic role of anti‐DNA antibodies, but studies on normal cells are limited. Here, we used the monoclonal anti‐dsDNA antibody 2C10, 2‐kbp dsDNA and healthy human peripheral blood mononuclear cells (PBMCs) to test ...
anti-DNA antibodiesELISABackground: Anti-dsDNA antibodies frequently found in the sera Systemic Lupus Erythematosus patients, particularly in active disease stage. Nowadays exploit different eukaryotic and prokaryotic dsDNA as antigen source and different reagents as binder. The aim of this study to ...
In some embodiments, the anti-dsDNA/chromatin antibody and at least one additional antibody are recognized by different secondary antibodies (e.g., with different labels). Further provided are kits for determining the amount of a sample (test) antibody that specifically binds dsDNA and/or ...
Moreover, it is also important to mention that quantitative levels of anti-dsDNA antibodies in the samples were not provided but only the measured electrochemical currents. More recently, another electrochemical biosensor prepared by constant potential deposition of dsDNA onto SPCE has been reported for...
We hypothesize that this might be explained by the presence of anti-nucleosome antibodies, which can be detected by dsDNA/nucleosome ELISA or ENA-immunoblot. Clinicians should be aware of lower sensitivity (higher chance of false negatives) in EliA when interpreting anti-dsDNA test results. ...
These results established a simple model to demonstrate that anti‐DNA antibodies can cause dysregulation of cytokine network mimicking systemic lupus erythematosus in culture of normal PBMCs, and emphasize again the importance of maintaining anti‐DNA antibodies at low levels by treatment.K. Inoue...
Our results suggest that simple and reliable ELISA for high-avidity anti-dsDNA antibodies is the test of good clinical utility for the assessment of global SLE activity.doi:10.1007/s10067-013-2330-3Sladjana AndrejevicIvica JeremicMirjana Sefik-Bukilica...
Objective Anti-dsDNA antibodies (anti-dsDNA) are a component of all classification schemes in SLE and comprise one of the domains in validated activity indices. Anti-dsDNA is frequently measured commercially by an enzyme immunoassay (EIA) or Crithidia luciliae immunofluorescence test (CLIFT). To ...
Compagno M, Jacobsen S, Rekvig OP, et al. Low diagnostic and predictive value of anti-dsDNA antibodies in unselected patients with recent onset of rheumatic symptoms: results from a long-term follow-up Scandinavian multicentre study . Scand J Rheumatol 2013; 42 :311–16....