Background Antibodies to dsDNA are reported to be specific for lupus and are included in the main classification criteria, although pitfalls when used as a 'screening' test in patients with multiple medical symptoms have been described. Lupus is typically diagnosed in the first 50 years of life ...
Positivity for anti-dsDNA antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). In the present study, the significance of ELISA positive and Crithidia luciliae immunofluorescence test (CLIFT) negative anti-dsDNA sera was evaluated. There were 371 consecutive serum samples submitted ...
The anti-dsDNA ELISA is not used for routine purposes in our institute since it is flawed by false-positive results due to binding of negatively charged... MN Hylkema,H Huygen,C Kramers,... - 《Journal of Immunological Methods》 被引量: 91发表: 1994年 Frequent False-positive results of ...
we propose the following potential molecular mechanism of disease pathogenesis: anti-TIF1 autoantibodies reduce the ability to restrict viral infection, which leads to either increased susceptibility to a wider diversity of viral pathogens or increased...
extension time and the number of cycles were tested, with all of them yielding infectious virus. The relative efficiencies of different CPER conditions and the identity of CASV in transfected C6/36 cells were shown by immunofluorescence assay (IFA) using an anti-CASV monoclonal antibody (Fig.4...
Correlating the fraction of off-target zOTU abundance to the amount of 16S rDNA copies found in each sequencing well further confirmed this, being significantly anticorrelated (P=3e−5, rho=− 0.52, Fig. 1b). Further, the samples derived from mice held under germ-free conditions, and ...
The number of CD34+ glomerular capillaries in GL mice were significantly and negatively correlated with sBUN, sCr, uACR, serum levels of anti-dsDNA ab, glomerular size, glomerular cell number, glomerular damage score, and infiltrations of B220+ B-cells, CD3+ T-cells, and Iba1+ macrophages ...
were collected and rinsed by PBS. Cells resuspended by PBS were incubated by Alexa Fluor 488 anti-human EGFR (5ul/test; BioLegend, #352908, lot: B332782) for 15 minutes at room temperature. Alexa Fluor 488 Mouse IgG1, κ Isotype Ctrl (FC) Antibody (5ul/test; BioLegend, #400129,...
Subsequently, the slides were sequentially incubated with biotinylated goat anti-rabbit immunoglobulin at a concentration of 1 : 100 for 30 min at 37 °C and then reacted with a streptavidin–peroxidase conjugate for 30 min at 37 °C and 3ʹ-3ʹ diaminobenzidine as a chromogen substrate. ...
Of these 83 participants, 5 (6%) had a negative serum IgG anti-CCP2 test result using the Immunocap kit (Phadia) (Table 1) at the time of periodontal assessment. Periodontal Assessment The percentage of periodontal sites with CAL 2 mm or greater, PPD 4 mm or greater, BOP, PDD, and ...