The methyl- CpG -binding domain protein MBD4 enzymatically removes T or U from the mismatched sites and protects against tumorigenesis (Millar CB et al 2002 Science 297:403). Thymine DNA glycosylase (TDG) has similar repair function. Sodium bisulfite converts cytosine but not 5mC into uracil....
DNA methylation is mostly found on cytosines within CpG dinucleotides10, and thus C > T transitions driven by 5mC deamination preferentially accumulate at CpGs (CpG > TpG mutations). In humans, two BER glycosylases are implicated in the repair of G:T mispairs, MBD4 (Methyl-CpG ...
A simple assay for monitoring the mutagenic effects of 5-methyl- cytosine deamination in Escherichia coli. Mutat. Res., in press.Petropoulos, L., Vidmar, J. J., Passi, E., and Cupples, C. G. (1994) A simple assay for monitoring the mutagenic effects of 5-methylcytosine deamination in...
The methyl group added to the cytosine ring at the N5 position is biologically stable. The carbon-carbon bond between the cytosine ring and the methyl group has high bond strength, especially in biological systems. Although the bond is stable, the positioning and abundance or 5mC are dynamic ...
(1994), 'Effect of DNA cytosine methyl- ation upon deamination-induced mutagenesis in a natural target sequence in duplex DNA', J. Biol. Chem. Vol. 269, pp. 7066–7069. 19. Pfeifer, G.P. (2006), 'Mutagenesis at methylated CpG sequences', Curr. Top. Microbiol. Immunol. Vol. 301, ...
TheoreticalStudyontheHydrolysisReactionMechanismof5一methylcyt0sine ZhangAihua ,YangBaohua,LiLin (YanjingMedicalCollege,CapitalMedicalUniversity,Beijing101300,China) Abstract:Thehydrolysisreactionmechanismandpotentialenergysurfaceof5-methylcytosine(mC)wereinves— ...
Valinluck, V., and Sowers, L.C. (2007). Endogenous cytosine damage products alter the site selectivity of human DNA maintenance methyltransferase DNMT1. Cancer Res 67, 946-950. Valinluck, V., Tsai, H.H., Rogstad, D.K., Burdzy, A., Bird, A., and Sowers, L.C. (2004). Oxidative...
DNA deaminases of the Aid/Apobec family convert cytosine into uracil and play keyroles in acquired and innate immunity. Epigenetic modification by methylat... HD Morgan,W Dean,Heather,... 被引量: 855发表: 0年 Deamination of 5-Methylcytosines within Cyclobutane Pyrimidine Dimers Is an Important...
29. Valinluck, V., and Sowers, L.C. (2007). Endogenous cytosine damage products alter the site selectivity of human DNA maintenance methyltransferase DNMT1. Cancer Res 67, 946-950. 30. Valinluck, V., Tsai, H.H., Rogstad, D.K., Burdzy, A., Bird, A., and Sowers, L.C. (2004...
Cytosine and 5-methylcytosine react with osmium tetraoxide in the presence of 2,2′-bipyridyl or N,N,N′,N′-tetramethylethylenediamine to form oxoosmium (IV) esters by addition to the 5,6-double bond. These esters react with water to form the corresponding uracil and thymine derivatives by...