Deamination of 5-Methylcytosines within Cyclobutane Pyrimidine Dimers Is an Important Component of UVB Mutagenesis.Studies the mutagenic consequences that are associated with the deamination of cytosine within cyclobutane pyrimidine dimers (CPDs), in particular with respect to the involvement of 5-...
The mechanism for the deamination reaction of 5-methylcytosine with H2O in protic medium was investigated using DFT calculations at the B3LYP/6-311G(d,p) level of theory. Two pathways were found. Pathway 5mA is a two-step mechanism where the N3-protonated 5-MeCyt undergoes a nucleophilic ...
This hypermutability is related to its role as the major site of cytosine methylation with the attendant risk of spontaneous deamination of 5-methylcytosine (5mC) to yield thymine. Cytosine methylation, however, also occurs in the context of CpNpG sites in the human genome, an unsurprising ...
5,6‐dihydro‐5‐methylcytosine5,6‐dihydrocytosinebisulfite conditiondeaminationThe discrimination of DNA methylation could make more complicated with the appearance of these minor bases. The conventional bisulfite sequencing faces with the tremendous challenge in the discrimination of DNA methylation. ...
Man's yesterday may ne'er be like his morrow; Nought may endure but Mutabililty.Percy Bysshe Shelley (1816) MutabilityThe first hint that the cytosine-guanine (CpG) dinucleotide might constitute a hotspot for pathological mutations in the human genome ca
5-Methylcytosine residues are deaminated to thymine, which cannot be excised and repaired by this system. As a result, 5-methylcytosine residues are hotspots for spontaneous transitions, as demonstrated in the lacI gene of Escherichia coli. We show here that in bacteria which lack uracil-DNA ...
the deamination of 5-methylcytosine, p53 mutations were also detected in sporadic colon cancer tissue and were associated with iNOS activity in these tissues... JE Goodman,LJ Hofseth,SP Hussain,... - 《Environmental & Molecular Mutagenesis》 被引量: 131发表: 2010年 Evolution of Phosphorylation-De...
Hydroxylation of 5-Methylcytosine by TET1 Promotes Active DNA Demethylation in the Adult Brain Summary Cytosine methylation is the major covalent modification of mammalian genomic DNA and plays important roles in transcriptional regulation. The molec... Junjie U.,Guo,and,... - 《Cell》 被引量: ...
We report that deamination coupled with 5-carboxyvinyl-2'-deoxyuridine-mediated photoligation causes the heat-induced transition of 5-methylcytosine to thymine with high selectivity and efficiency without any side reaction. The differenc... M Takashi,Y Yoshinaga,F Kenzo - 《Nucleic Acids Symposium》...
5-Hydroxymethylcytosine (5hmC) binding, ES-cell-specific (HMCES) crosslinks to apurinic or apyrimidinic (AP, abasic) sites in single-strand DNA (ssDNA). To determine whether HMCES responds to the ssDNA abasic site in cells, we exploited the activity of apolipoprotein B mRNA-editing enzyme...