一、10x Genomics单细胞多组学ATAC+基因表达技术原理 为同时获得同一细胞的转录组和表观组,10x Genomics单细胞多组学ATAC+基因表达技术首先利用Tn5转座酶对细胞核悬液进行核DNA转座,Tn5转座酶优先切割开放染色质区域中的核DNA。然后利用Chromium平台进行单细胞基因表达(GEX)和ATAC文库制备,该过程将单个细胞核、反应试剂与...
adata_atac = sc.read_10x_mtx('outs/filtered_feature_bc_matrix/', var_names='gene_symbols', cache=True, gex_only=False) adata_atac = adata_atac[:,adata_atac.var['feature_types'] == "Peaks"] # We aggregate peaks around each gene as well as those that have high correlations with p...
美国纽约基因组中心的研究人员开发了两种单细胞多组学检测方法,一种是借助10x scATAC试剂盒,在单细胞水平同时检测染色质可及性、蛋白水平和线粒体DNA,命名为ASAP-seq (ATAC with select antigen profiling)。在文章的审稿过程中,10x推出了GEX+ATAC的单细胞多组学试剂盒,作者在此基础上又开发了一种新方法Dogma-seq,...
cellranger ARC 【联合分析RNA和ATAC,multi-omics】 参考: 单独ATAC 10x的单细胞ATAC上游流程之cellranger-atac 联合分析RNA+ATAC What is Cell Ranger ARC?- 官网 最新单细胞多组学新产品:ATAC + 基因表达- 【基本原理】 单细胞多组学ATAC- Cell Ranger ARC--count 单细胞多组学ATAC-Cellranger ARC-结果解读之...
Chromium X(简称X平台)可以在一次运行中分析数百到数十万细胞,实现更多细胞的检测,是目前10x Genomics推出的最高通量的仪器。X平台与10x单细胞转录组、单细胞免疫组库(VDJ)、单细胞ATAC、单细胞多组学ATAC+GEX等多种应用兼容。 那么X平台具体能做什么?
10x Genomics Cloud Web applications Cloud Analysis Analyze 10x Genomics data quickly and securely in the cloud. Currently compatible with Single Cell Gene Expression, Single Cell Immune Profiling, and Single Cell Multiome ATAC + GEX products. ...
atac: { ... } gex: { ... } dimensionality_reduction: atac: { ... } gex: { ... } feature_linkage: ... tf_analysis: ... - Run summary HTML: /home/jdoe/runs/sample345/outs/web_summary.html - Run summary metrics CSV: /home/jdoe/runs/sample345/outs/summary.csv ...
精彩回顾 1. Cell Ranger 知多少(下) 2. 如果要做单细胞ATAC,那你一定要了解这款分析工具 3. 单细胞ATAC分析利器ArchR教程第三篇—Pseudo-Bulk 水平分析 4. 探索单细胞测序中的恶性肿瘤细胞,一定不能少了它——inferCNV 点击查看更多精彩内容~
Loupe Browser is a desktop application for Windows and MacOS that allows you to quickly and easily visualize and analyze 10x Genomics Chromium™ Next GEM Single Cell Multiome ATAC + Gene Expression (GEX) data. It combines the tools previously developed for looking atSingle Cell Gene Ex...
Can I share projects on 10x Genomics Cloud Analysis? Why do I have an error "Sequence and quality not separated by +" while running 10x Genomics Cloud Analysis? What parameters can a user specify for multiome ATAC and GEX samples in 10x Genomics Cloud Analysis? What parameters can a user ...