无翅型MMTV整合位点家族成员5A(WNT5A)重组蛋白Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES! Organism species Homo sapiens (Human) Product No. XY549Hu01 Source Prokaryotic expression Host ...
The expression of inflammatory mediators and metalloproteases was analysed by real-time PCR after stimulation with recombinant Wnt5a protein (rWnt5a), TNF and combined treatment. Results We analysed the effect of the absence of Wnt5a and rWnt5a on the proliferation, migration and invasion of RA ...
To assess the spheroid formation ability of C9/SKOV-3 clone versus mock 1.5 × 104 cells were cultured in 6-well plates coated with 1% low melt agarose (IBI SCIENTIFIC, Tryon, NC, USA) in serum-free RPMI supplemented with 20 ng/ml recombinant human epithelial growth factor and 20 ng...
Recombinant Wnt5a was purchased from R&D Systems. DAPT was purchased from Sigma-Aldrich (St Louis, MO, USA). The antibodies used in this study include anti-Ryk (Abgent, San Diego, CA, USA), anti-Vangl2 (Santa Cruz Cell Discovery | www.nature.com/celldisc Biotechnology, Dallas, TX, USA...
Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)Signal transduction Product No.RPP549Hu01Organism SpeciesHomo sapiens (Human) Same name, Different species. SourceProkaryotic expressionHostE.coliEndotoxin Level<1.0EU per 1µg (determined by the LAL method)...
After optimization of the IHC assay, all four antibodies showed cytoplasmic WNT5A expression in tissue samples; in contrast, only one antibody detected WNT5A in western blots. A pre-absorption test with recombinant WNT5A showed that AF645 and 3A4 antibodies specifically detected WNT5A in different...
and plated at 1.5 × 106/well/mL in a 24-well tray on a layer of irradiated HeLaCD154 cells (80 Gy) at a CLL/HeLaCD154 cell ratio of 15:1 in complete RPMI 1640 medium supplemented with 5 ng/mL recombinant human IL-4 (R&D Systems), and 15 ng/mL recombinant human IL-10 (R&D ...
primary human myometrial cells; (b) anti-WNT5A antibody reduced the stimulatory action of myometrial CCM on colony formation, self-renewal, and TCF/LEF transcriptional activities of eMSCs; and (c) recombinant WNT5A protein increased the formation of colonies and phenotypic expression of eMSCs....
Recombinant human/mouse Wnt5a was purchased from R&D Systems (Minneapolis, MN). 1α,25(OH)2D3 and PLAA were purchased from Enzo Life Sciences (Plymouth Meeting, PA). The anti-PLAA polyclonal antibody was designed and developed by Strategic Diagnostics Inc. (Newark, DE) [12]. Rabbit antiseru...
AIM: To understand how Ror2 overexpression in Dkk-1 secreting epithelia signals CDX2, villin, and SGLT1 and show recombinant human (rh) Dkk-1 interacts with Ror2 using epithelial cells which do not secrete Dkk-1 constitutively. METHODS: PCR cloning was used to construct His and Myc-tagged ...