WNT5A (Human) Recombinant Protein (P01) 供应商 Abnova 产品货号 H00007474-P01 产品报价 ￥询价/2ug 产品说明书 点击查看 购买方式 在线支付宝及网银支付、支票、现金、电汇,或拨打全国免费服务电话4006800868与我们联系。 产品新闻 背景资料 提供的WNT5A人源重组蛋白(P01)产品为带有GST标签的人源WNT5A全长序列...
To assess the spheroid formation ability of C9/SKOV-3 clone versus mock 1.5 × 104 cells were cultured in 6-well plates coated with 1% low melt agarose (IBI SCIENTIFIC, Tryon, NC, USA) in serum-free RPMI supplemented with 20 ng/ml recombinant human epithelial growth factor and 20 ng...
Corp.). NanoLuc is an artificially designed luciferase of 19 kDa, which is smaller than classical firefly- orRenilla-luciferases (62 kDa and 36 kDa, respectively). Because of smaller molecular weight, NanoLuc does not hinder the secretion of recombinant proteins with signal peptides for extra-ce...
靶点:Protein Wnt-5a 宿主:Rabbit 用途:仅用于科研 应用范围:WB,IHC,ELISA 纯度:0.95 % 产地:China 品牌:AtaGenix 货号:PA9765H 用途:仅用于科研 亚型:IgG 是否单克隆:否 免疫原:E. coli - derived recombinant Human WNT5A (Ile62-Lys380). 保质期:1 year ...
Relative to recombinant TxWT, we reasoned that there would be a delay in the toxigenic effects of S. Javiana due to the time needed to express and exocytose typhoid toxin from the host cell.21 We accounted for the delay by imaging infected cells at 96 h, at which point pSMAD2 had ...
Recombinant human/mouse Wnt5a was purchased from R&D Systems (Minneapolis, MN). 1α,25(OH)2D3 and PLAA were purchased from Enzo Life Sciences (Plymouth Meeting, PA). The anti-PLAA polyclonal antibody was designed and developed by Strategic Diagnostics Inc. (Newark, DE) [12]. Rabbit antiseru...
structure. In addition, our live imaging experiments studying F-actin dynamics show that Wnt5a, through HRI, also increases the F-actin mobility, mainly producing retraction, probably to ensure spine maturation. Here, we found that GluN2B is a novel postsynaptic target protein of Wnt5a, whose ...
Anti-Wnt5a antibodies were identified by recombinant protein-based panning according to the manufacturer's instructions. His-Wnt5a, which was expressed in and purified from E. coli, was captured on Maxisorp plates (Thermo Scientific, Waltham, MA) and incubated with pools of HuCAL Platinum phage....
For the pull-down assay, Fc- CD146/ECD, Fc-Fz5/ECD or Fc (100 ng ml À 1 each) was conjugated with protein A beads and incubated with recombinant Wnt5a for 1 h. Bound proteins were eluted with loading buffer and analysed by immunoblotting. Full scans of western blots are supplied ...
1B). HFD increased Wnt5a mRNA and protein levels in subcutaneous WATs more so than in epididymal WATs (Fig. 1C, D), and HFD for 35 weeks increased Wnt5a expression more than HFD for 8 weeks (Fig. 1C). However, HFD did not increase Wnt5a expression in livers, hindlimb muscles, or...