Methods:CCK 8 was used to detect the effect of different concentrations of recombinant protein Wnt5a on HaCaT cell proliferation, flow cytometry assay was used to detect the effect of exogenous Wnt5a on HaCaT cell cycle and Ca2+ concentrations. Then HaCaT cells were stimulated by Wnt5a and Friz...
The expression of inflammatory mediators and metalloproteases was analysed by real-time PCR after stimulation with recombinant Wnt5a protein (rWnt5a), TNF and combined treatment. Results We analysed the effect of the absence of Wnt5a and rWnt5a on the proliferation, migration and invasion of RA ...
After optimization of the IHC assay, all four antibodies showed cytoplasmic WNT5A expression in tissue samples; in contrast, only one antibody detected WNT5A in western blots. A pre-absorption test with recombinant WNT5A showed that AF645 and 3A4 antibodies specifically detected WNT5A in different...
On the basis of the previous report that WNT5A promotes the expression of cytokines and chemokines in human cells (Rauner et al, 2012; Li et al, 2014; Zhao et al, 2014b), androgen-sensitive human CaP cells were treated with recombinant WNT5A protein. In 22Rv1 and LNCaP cells, Wnt5A...
Spheroid formation assay To assess the spheroid formation ability of C9/SKOV-3 clone versus mock 1.5 × 104 cells were cultured in 6-well plates coated with 1% low melt agarose (IBI SCIENTIFIC, Tryon, NC, USA) in serum-free RPMI supplemented with 20 ng/ml recombinant human epithelial ...
34 All typhoid toxin subunits were identified in our input material of recombinant TxWT (Figure S2H), which was consistent with the reported 1 CdtB:1 PltA:5 PltB stoichiometry.22 However, no peptides for typhoid toxin subunits CdtB or PltA were identified in the proteomic analysis of ...
(a) WNT5A was among the highly expressed WNT ligands in primary human myometrial cells; (b) anti-WNT5A antibody reduced the stimulatory action of myometrial CCM on colony formation, self-renewal, and TCF/LEF transcriptional activities of eMSCs; and (c) recombinant WNT5A protein increased ...
Recombinant Human Thioredoxin-Like ... Monoclonal antibody to Human PD-L1 ... Monoclonal Antibody to Human IL-1be... Monoclonal Antibody to Caspase-3 (P... Polyclonal Antibody to Beta actin Monoclonal Antibody to GAPDH (Clone... Monoclonal antibody to B7-H4 (Clone... Polyclonal Antibody to...
These fibroblasts were transfected with si-RNA-EGFR (si-EGFR) or si-RNA-scramble (si-Scram) for 48 h and then treated with recombinant rat Wnt5a (rWnt5a) or Wnt11 (rWnt11), respectively, 30 min later, these cells were analyzed. n = 5/group. All data were shown as mean...
6i, j). Treating of PDAC cells with recombinant protein or neutralizing antibody revealed that Pcsk9, but not PGRN, increased PDAC proliferation under glucose-limiting conditions, indicating the role of Pcsk9 in regulating tumor growth (Fig. 6k, l). To further validate the function of Pcsk9,...