The MA5-15511 immunogen is purified recombinant fragment of WNT5A expressed in E. Coli. . MA5-15511 detects WNT5A which has a predicted molecular weight of approximately 42.3kDa. 靶标信息 Wnt5A is a secreted protein found in the extracellular region that belongs to the Wnt family. It is ...
In our study, we found that recombinant Pcsk9 protein could decrease the protein level of Ldlr (Supplementary Fig. 10a). In addition, increase LDL-C level was able to sustain the PDAC survival in vitro in response to low-glucose treatment (Supplementary Fig. 10b). Overexpression of Ldlr ...
As shown in Fig.1A, we first found overexpression ofWNT5AmRNA and Wnt5a protein in HTLV-1-related T cell lines. Most ofWNT5AmRNA levels were more than 10 times higher in HTLV-1 infected immortal cell lines (MT-2 and C91/PL), and ATL patient-derived T-cell lines (HUT102, MT-1, ...
The involvement of WNT/β-catenin signaling in self-renewal of eMSCs was confirmed with the use of WNT activator (Wnt3A conditioned medium) and WNT inhibitors (XAV939 and inhibitor of Wnt Production-2 [IWP-2]). The myometrial cells expressed more WNT5A than other WNT ligands. Recombinant ...
1B). HFD increased Wnt5a mRNA and protein levels in subcutaneous WATs more so than in epididymal WATs (Fig. 1C, D), and HFD for 35 weeks increased Wnt5a expression more than HFD for 8 weeks (Fig. 1C). However, HFD did not increase Wnt5a expression in livers, hindlimb muscles, or...
However, the effect of SFRP5/WNT5A axis on recombinant human interleukin-13 (rhIL-13)-induced inflammation has not been studied. In this study, we aimed to investigate whether secreted frizzled-related protein 5 (SFRP5) could modulate the production of cytokines relevant to eosinophil infiltration...
protein. Although this study appears to be contradictory with our data, we described an active role for Wnt5a increasing GluN2B levels by translational de-repression through NO production and HRI kinase activation. Instead, the complete absence of Wnt5a might not decrease GluN2B subunits because, ...
the tumourigenic characteristics of WNT5A is further supported as it stimulates production of inflammatory cytokines and chemokines in endothelial cells, monocytes, bone stromal cells, and cancer cells (Rauner et al, 2012; Jung et al, 2013; Li et al, 2014) via mitogen-activated protein kinase...
Although purified Wnts are useful tools to better understand the roles of Wnt signaling in the inflammatory responses, it has been reported that recombinant Wnt preparations contain TLR agonists that may lead to inflamma- tory cytokine production14. Therefore, further in vivo studies elucidating the...
Consistent with a conserved role for TGF-β ligands in paracrine senescence and their secretion in response to APH and ETP (Figures 1H and S3F), APH and ETP triggered pSMAD2 production, which was also observed with the positive control TGF-β3 at 48 h (Figures 3B and 3C). The same ...