Wnt5A is a secreted protein found in the extracellular region that belongs to the Wnt family. It is a proto-oncogene that functions in modulating cell fate and behavior during vertebrate development and has an important role in Wnt signaling pathway. It is a glycoprotein that plays a...
Wnt5a knockdown cells, after the recombinant lentivirus infection. The % Venus (+) cells in shWNT5Aexpressing cells decline rapidly during infection, while that with shLUC(the negative control) does not. The induction of cell-death in Wnt5a knockdown cells were more significant in MT-2 and TL...
Recombinant mouse Wnt5a, Wnt3a, Wnt7a, Wnt 9b and Wnt10b were purchased from R&D systems Co. Ltd. (Minneapolis, MN). Chemicals and reagents used in this study were of analytical grade. Cell cultures. Calvarial cells were prepared from the calvariae of Wnt5a2/2 and wild- type embryos at...
The MA5-15511 immunogen is purified recombinant fragment of WNT5A expressed in E. Coli. . MA5-15511 detects WNT5A which has a predicted molecular weight of approximately 42.3kDa. 靶标信息 Wnt5A is a secreted protein found in the extracellular region that belongs to the Wnt family. It is ...
The tumour-suppressive effects of transforming growth factor-beta (TGF-β) are well documented; however, the mechanistic basis of these effects is not fully understood. Previously, we showed that a non-canonical member of the Wingless-related protein fam
Secreted frizzled‐related protein 5 serum levels in human periodontitis—A nested case–control study sFRP5wnt5aAim Recombinant secreted frizzled‐related protein 5 ( sFRP 5) improved periodontal status in mice. Thus, this study aimed to investigate this ... J Schulz,C Knappe,C Graetz,... -...
The mTOR signaling pathway is a key mechanism by which synaptic activity stimulates protein synthesis in neurons [38, 39]. However, our results indicate that this pathway is not involved in the activation of NMDAR-regulated Wnt5a mRNA translation (Figure 4A, B). Instead, the NMDAR-elicited Wnt...
Recombinant human/mouse Wnt5a was purchased from R&D Systems (Minneapolis, MN). 1α,25(OH)2D3 and PLAA were purchased from Enzo Life Sciences (Plymouth Meeting, PA). The anti-PLAA polyclonal antibody was designed and developed by Strategic Diagnostics Inc. (Newark, DE) [12]. Rabbit antiseru...
expressed as relative units. Lines represent mean ± SEM. N = 4 (*p = 0.0011;p = 0.0288;p = 0.0245) (d) Representative blot showing hippocampal neurons treated with 300 ng/mL recombinant Wnt5a (rW5a), rW5a+CHX or CHX. The plot shows the GluN2B expression relative...
This could be validated at the protein level using both small interference RNA and recombinant Wnt5A (rWnt5A). Among the genes up-regulated by WNT5A was the gene vimentin, associated with an epithelial to mesenchymal transition (EMT), which involves decreases in E-cadherin, due to up-...