2019.10.07 Compared with other buffers such as PBS (phosphate buffered saline) and TRIS, HEPES has higher stability in maintaining the pH values of the cell culture media, that’s also the reason why HEPES is widely used in cell culture Latest Entries MES Buffer: An Essential Component in ...
If the Balkans seem complicated and confusing, that is because they are. But there is nothing incomprehensible or even arcane about the driving factors, which exist elsewhere as well. War in the Balkans, as in many other parts of the world, is politics by other means. Distribution of power ...
AtWhy2 is associated with mtDNA and its overexpression results in the production of dysfunctional mitochondria. This report constitutes the first evidence of a function for the Whirlies in organelles. We propose that they could play a role in the regulation of the gene expression machinery of orga...
First, the pathophysiological origin of the damage (e.g. presence of comorbidity) can dramatically alter the circulation dynamics of a given organ injury/function parameter(s) in the CLP model (and sepsis in general). Second, even when the elevation of those parameters is very high, it ...
Samples were then immersed in permeabilization buffer (0.5% TX-100, 10% FBS in PBS) for 30 min at rt, followed by three washes in stain buffer. Samples were then exposed to stain buffer containing both primary antibodies (abs) each in 1:200 concentrations over night at 4°C rocking on ...
The Balkans region is a good place to learn that and other lessons. Some readers will doubt that the interventions in the Balkans have any claim on success. To them I recommend reading the quantitative analysis offered by RAND.23 Of the twenty multilateral interventions since 1989, Bosnia and ...
The Balkans region is a good place to learn that and other lessons. Some readers will doubt that the interventions in the Balkans have any claim on success. To them I recommend reading the quantitative analysis offered by RAND.23 Of the twenty multilateral interventions since 1989, Bosnia and ...
How To Be Creative: PBS’ Off Book Series Explores the Secret Sauce of Great Ideas Josh Jonesis a writer and musician based in Durham, NC. Follow him at@jdmagness
Very recently, we confirmed 5-iodo-4-thio-2 -deoxyuridine (ISdU) as an efficient agent, increasing the extent of tumor cell killing with ionizing radiation. To our surprise, a similar derivative of 4-thio-2'-deoxyuridine, 5-bromo-4-thio-2 -deoxyuridine (BrSdU), does not show radio...