In short, all of this business has helped buffer the industry against declines in other core satellite market sectors and masked the economic effects of the proliferation of cheap terrestrial bandwidth. As we all know, the political winds are changing and the pressure to pull the troops out ...
Phosphate Buffer SolutionHybrid MaterialThermal Gravimetric AnalysisOsteoproductive materials that induce quick bone regeneration are needed for the developing area of scaffold-based bone engineering. Bioactive silica-based glasses, ceramics, and hybrids are called to play an important role in this field. ...
2.5. Histology, calculation of wall thickness and cross-sectional area Following relaxation in Ca2+-free Krebs, arterial segments adjacent to those used in circumference–tension experiments were fixed with 2% formaldehyde in phosphate-buffered saline (PBS) (30 min). Arteries were then washed in PBS...
To ensure the homogenization process did not introduce contamination, we collected one blank subsample of sterile PBS buffer in which a new homogenization probe had been inserted and pulsed, and one blank subsample of sterile PBS buffer alone. These blanks were treated as samples and processed ...
The supernatant (0.7 mL) was mixed with 0.7 mL of 10 mM phosphate buffer (pH 7.0) and 1.4 mL of 1 M potassium iodide. After 1 h of reaction in the dark, the absorbance of the supernatant at 390 nm (X) was measured using a spectrophotometer. The H2O2 content (Y) was calculated ...
Erythrocytes were pelleted by centrifugation at 1000 g for 15 min, and were washed and centrifuged three times in cold PBS buffer (140 mM NaCl, 2.7 mM KCl, 1.5 mM KH2PO4, 8.1 mM Na2HPO4·2H2O, pH 7.4) until the supernatant was clear and colorless. Two mL of packed cells were then...
samples was done as per the ‘blood and body fluid protocol’ of Qiagen Blood mini kit, Germany with slight modifications which includes treatment with lysis buffer for 30 minutes at 56oC, extended treatment with ethanol for 20 minutes and final DNA elution in 50-75µl of TE buffer. In...
The protocol was established by optimizing the concentrations of the coating antigen (pre-F proteins), secondary antibodies, and blocking buffer. To validate the protocol, we examined its accuracy, precision, and specificity using serum samples from 150 participants across various age groups and the ...