To ensure the homogenization process did not introduce contamination, we collected one blank subsample of sterile PBS buffer in which a new homogenization probe had been inserted and pulsed, and one blank subsample of sterile PBS buffer alone. These blanks were treated as samples and processed ...
Phosphate Buffer SolutionHybrid MaterialThermal Gravimetric AnalysisOsteoproductive materials that induce quick bone regeneration are needed for the developing area of scaffold-based bone engineering. Bioactive silica-based glasses, ceramics, and hybrids are called to play an important role in this field. ...
When working with free sulfhydryls, EDTA is generally included in the buffer system for its antioxidative effect. EDTA chelates trace amounts of metals in solution that promote disulfide bond formation. Using nitrogen-purged buffers is an additional precaution to prevent oxidation of the free ...
2. Pour off the media and suspend the cell pellet in 0.5-1 ml of cold 1X PBS, pH 7.4, or buffer of choice. 3. Sonicate the cell pellet on ice. 4. We recommend doing a protein determination on the sample to normalize the PA concentration to protein concentration (nmol PA/mg protein...
sEV samples were lysed in ice-cold RIPA buffer (Beyotime, China) on ice for 15 min and centrifuged at 13,000 g for 10 min. The protein concentration in the supernatant was determined via the BCA assay (Pierce, NCI225CH). Thirty micrograms of total protein was separated by 12% so...
2.5. Histology, calculation of wall thickness and cross-sectional area Following relaxation in Ca2+-free Krebs, arterial segments adjacent to those used in circumference–tension experiments were fixed with 2% formaldehyde in phosphate-buffered saline (PBS) (30 min). Arteries were then washed in PBS...
The supernatant (0.7 mL) was mixed with 0.7 mL of 10 mM phosphate buffer (pH 7.0) and 1.4 mL of 1 M potassium iodide. After 1 h of reaction in the dark, the absorbance of the supernatant at 390 nm (X) was measured using a spectrophotometer. The H2O2 content (Y) was calculated ...
concentrations of the SPF extract dissolved in DMSO, and DMEM and DMSO were used as references. The L02 cells were continued to be treated for an additional 24 h, the supernatants were removed, and PBS was used to wash the cells 3 times. The cell viability was measured using the MTT ...
samples was done as per the ‘blood and body fluid protocol’ of Qiagen Blood mini kit, Germany with slight modifications which includes treatment with lysis buffer for 30 minutes at 56oC, extended treatment with ethanol for 20 minutes and final DNA elution in 50-75µl of TE buffer. In...
2. Pour off media and add 2 ml of PBS (pH 7.2-7.4) to wash cells. 3. Centrifuge at 1,000-2,000 x g for 10 minutes at 4°C. 4. Sonicate cell pellet in 0.5-1 ml of cold buffer (i.e., PBS, pH 7.2-7.4, containing a pan protease inhibitor cocktail; i.e., Roche Item No...