Bacteria have evolved diverse mechanisms to fend off predation by bacteriophages. We previously identified the Dnd system, which uses DndABCDE to insert sulfur into the DNA backbone as a double-stranded phosphorothioate (PT) modification, and DndFGH, a restriction component. Here, we describe an un...
Choose the right Western blot blocking buffer and get reduced background interference, increased signal-to-noise ratio, and minimal non-specific binding.
parameter status disp parameter and variabl parameter buffer addr parameter family parameter mode displa parameter number too parameter performance parameter random acce parameter segment des parameter spacebr parameterize method parametereffective st parametric acoustic a parametric coords parametric drive paramet...
Harvested cells were washed twice in PBS and resuspended for 10 min on ice in buffer A (10 mM Hepes, pH 7.9 at 4 °C, 1.5 mM MgCl2, 10 mM KCl and 0.5 mM DTT). Cells were centrifuged at 2000, r.p.m. for 5 min at 4 °C. Cell pellet was resuspended in b...
Lysinibacillus sphaericus C3-41 carries a large low-copy-number plasmid pBsph, which encodes binary toxin proteins. Our previous study found that the transcriptional activator TubX plays an important role in the newly identified type Ⅲ TubRZC replication/partition system in pBsph, and that a vect...
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Proaerolysin was diluted into a buffer containing 50 mm Tris acetate, 1 mmCaCl2, pH 7.4, to a final concentration of 2.8 μm and incubated for 60 min at 37 °C with a given volume of partially purified enzyme. The corresponding enzymatic activity on the fluorogenic peptide (see above), ...
EXT_color_buffer_half_float;EXT_frag_depth;EXT_shader_texture_lod;EXT_texture_filter_anisotropic;WEBKIT_EXT_texture_filter_anisotropic;EXT_sRGB;OES_element_index_uint;OES_standard_derivatives;OES_texture_float;OES_texture_float_linear;OES_texture_half_float;OES_texture_half_float_linear;OES_vertex_...
p53 is a tumor suppressor, which belongs to the p53 family of proteins. The family consists of p53, p63 and p73 proteins, which share similar structure and function. Activation of wild-type p53 or TAp73 in tumors leads to tumor regression, and small molecules restoring the p53 pathway are ...
USA) with the RNA 6000 Nano LabChip kit. During the in vitro transcription process, 1 μg of total RNA was amplified by a low RNA input fluor linear amp kit (Agilent) and labeled with Cy3 (CyDye, PerkinElmer, Waltham, MA, USA). Using incubation with fragmentation buffer at 60 °...