The most common composition of PBS (1X) has the following components: Salt Concentration (mmol/L) NaCl 137 KCl 2.7 Na2HPO4 10 KH2PO4 1.8 The above buffer solution may give the approximate ionic concentration of 10 mM HPO4 -2 , 157 mM Na+,...
PBS buffer pH 7.5 Concentration 1mg/ml Purity >95% Clonality Monoclonal Isotype IgG1-kappa Applications Research Grade Isatuximab Biosimilar Target Cyclic ADP-ribose hydrolase 1,ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1,cADPr hydrolase 1,2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP...
For affinity-precipitation, proteins (~5 mg) were solubilized from five dishes (round, 10 cm in diameter) of the heavy- and light-labeled cells, respectively, with a lysis buffer containing 2% dodecyl maltoside in 150 mM NaCl and 20 mM Tris-HCl (pH 7.4). The solubilized proteins...
NDVEFTWLRGNDSVRGLEHLRLAQYTIERYFTLVTRSQQETGNYTRLVLQFELRRNV 蛋白标签: N-terminal 6xHis-tagged 产品提供形式: Liquid or Lyophilized powder 缓冲液: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder,...
Then, the supernatant was removed, cells were washed twice with ice-cold PBS and harvested by scraping directly into lysis buffer (100 mM NaCl, 50 mM HEPES-NaOH pH 7.9, 1 mM EDTA, 5% glycerol, 0.05% NP-40, 0.1% SDS, proteases and phosphatases inhibitors − 10 mM sodium fluoride (...
A total of 1 × 106 control or ALKBH5 knock-down U87 GBM cells were resuspended in 200μL PBS (pH = 7.4) and injected into the backs of nude mice subcutaneously. The tumor size was measured every five days for each mouse when tumor growing. The nude mice were euthanized at ...
AntiFix™ Universal Antigen Retrieval Buffer has been formulated to provide excellent heat unmasking for a wide variety of targets at neutral pH, reducing the need for buffer optimization. It has been validated in immunofluorescence staining of FFPE tissue with our next-generation fluorescent CF®...
Prior to PAGE, CSF samples were added to a mixture containing 4× LDS sample loading buffer (2% lithium dodecyl sulfate, 10% glycerol, 200 mM Tris; pH 8.4) and DTT (50 mM final concentration). The samples were diluted with PBS to ensure equal loading volumes and heated at 70 °C for...
Afterward, the embryos were washed 4 × 15 min in PBT followed by 3 × 15 min washes in alkaline phosphatase buffer (100 mM NaCl, 50 mM MgCl2, 100 mM Tris–HCl pH 9.5). The embryos were then transferred to 1 ml of alkaline phosphatase buffer and the in situ ...
Transient reprogramming by the expression of OCT4, SOX2, KLF4 and MYC (OSKM) is a therapeutic strategy for tissue regeneration and rejuvenation, but little is known about its metabolic requirements. Here we show that OSKM reprogramming in mice causes a g