**PBS (phosphate-buffered saline) is a common buffer used in cell culture and histology. The osmolarity and ion concentration of PBS are the same as in human tissues, so it will not cause your cells to shrivel or explode. PBS is 137 mmol NaCl, 2.7 mmol KCl, 10 mmol Na2HPO4, and 2 ...
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Recipes How To Make RIPA Lysis Buffer Recipes How To Make 1x Phosphate Buffered Saline (PBS) Recipes How To Make 5 M Sodium Chloride Solution 1 COMMENT Stephanie T January 23, 2020 At 4:21 pm Thanks for your LB media tutorial, I’m using it to create media in order to screen ...
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Then, single colonies were collected and used to prepare a suspension of LB broth containing 1 × 107 cells/ml, according to McFarland 1.0 standard optical density. The Petri dishes were stored at 4°C while fresh broth culture prepared prior to each experiment. 2.6. Staphylococcus aureus ...
10. Dilute the total protein to approximately 1 μg/μl with PBS to reduce the concentration of detergents in the lysis buffer. If the target protein is present at a low level in the cells, the total protein concentration should be slightly higher (e.g., 10 μg/μl). 11. Add a ce...
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Acid Safety Information You should always wear protective gear when mixing acid solutions. Be sure to wear safety goggles, gloves, and a lab coat as well. Tie back long hair and make sure your legs and feet are covered by long pants and shoes. It's a good idea to prepare acid solutions...
Decide on the pH for your buffer. This pH should be within one pH unit from the pKa of the acid/conjugate base. So, you can prepare a buffer at pH 2 or pH 7, for example, but pH 9 would be pushing it. Use theHenderson-Hasselbachequation to calculate how much acid and base you ...
How to convert the silica precursor to silica at a 优质文献 相似文献A simple way to prepare PbS nanocrystals with morphology tuning at room temperature A simple way to synthesize PbS nanocrystals with the ability to tune their morphology at room temperature is reported. The preparation utilizes an...