Ultrathin sections were transferred to nickel grids with formvar and immunolabeled. Grids were treated with blocking buffer containing 3% BSA in 0.01 M PBS buffer (pH 7.4), then incubated with a primary β-(1,3)-glucan specific antibody (mouse anti-1,3 beta-glucan [2G8] Abcam), then ...
Special attention should be paid to washing steps during an Immunofluorescence procedure, because Immunofluorescence (IF) quality can be increased by proper washing. PBS is a standard washing buffer, whereas variants such as PBS++or PBS-T are also prevalent. PBS++contains 1 mM CaCl2and MgCl2,which...
But in the case explained below, we expect the “SPIM:sclk_m” to drive at 24MHz. But with this configuration of Data Rate = 8MBPS and Oversampling of 6, we are getting clock rate of 4MPBS as shown in below pic as Actual Data Rate I,e 4000 KBPS, https://commu...
First we need to make some assumptions about the dataset and how frequently it will be accessed. Over the lifetime of the dataset we will assume that it will be written to twice, so 10PBs of written data. We will also assume that it will be read 10 times, and each object is an ...
Wash buffer: PBS Live/ dead cell stains: AM/ Propidium Iodide/ Hoechst 33342 (nuclear stain) Interested inorgan-on-chip?Make your experiment effortless thanks to our all-in-one pack! If the microfluidic chip used is non treated, and/or not sterile, here are two s...
I would wake up the decoder chip, wait for it to give the ESP some sort of "new frame" interrupt, read the data stream for exactly one frame and put the decoder to sleep. Then I would build e.g. JPEG from it (reshuffle the buffer I guess) and do whatever else with it. That's...
subtilis (strain Δhag amyE::Phag-hagT209C spec) was grown to an O.D. of 0.6, and 2 ml of the cells were pelleted via centrifugation and resuspended in 1 ml of PBS buffer pH 7.5. The 1 ml suspension was passed back-and-forth between two 5 ml syringes with 21 gauge ...
Decide on the concentration of the buffer. If you make up a concentrated buffer solution, you can dilute it as needed. Decide on the pH for your buffer. This pH should be within one pH unit from the pKa of the acid/conjugate base. So, you can prepare a buffer at pH 2 or pH ...
How Do You Make a Buffer? A buffer is made by mixing a large volume of aweak acidorweak basetogether with its conjugate. A weak acid and its conjugate base can remain in solution without neutralizing each other. The same is truefor a weak baseand itsconjugate acid. How Do Buffers Work?
Saline solution refers to a salt solution, which you can prepare yourself. The solution can be used as a disinfectant, sterile rinse, or for lab work.