A plasmid is a small, circular DNA molecule distinct from chromosomal DNA, possessing the unique ability to replicate independently. Essential in molecular biology, plasmids facilitate the manipulation, study, and plasmid DNA production of genes. Molecular cloning, a technique used to produce identical ...
What is a mutation that results in the addition of nucleotide pairs in a gene called? What is responsible for albinism in terms of DNA? What does DNA ligase do during DNA replication, and what is DNA ligase? What does DNA sequencing allow you to do in this activity?
What is Sanger sequencing? Explain how it works and how the PCR process is different. How does DNA polymerase facilitate the PCR reaction? Explain DNA amplification (PCR) and give one example of when it would be used. What are the steps in the PCR cycle and what is the purpose for each...
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Polymerase chain reaction (PCR) is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. These include DNA cloning for sequencing, DNA-based phylogeny, or functional analysis of genes, the diagnosis of hereditary diseases, and the...
In the aerobic atmosphere currently prevailing at the Earth’s surface, the most stable form of carbon is its fully oxidised state, CO2. The atmosphere contains 2.6 x 1012 tonnes of carbon as CO2 which is in equilibrium with even larger amounts (1.3 x 10
is the more extensively used tool for genome editing. However, the overwhelming efficiency in genome sequencing of different organisms has generated a large amount of data helping the identification of new systems whose use in genome editing is currently being explored. Among them there are new memb...
Thus, following this logic, when researchers design a gRNA sequence for their CRISPR experiments, they generally exclude the PAM from the guide RNA.This is especially important for plasmid-based systems, as the delivery format is DNA the region encoding the gRNA will be chopped by the Cas if ...
RIN (RNA integrity number) of RNA extracted from mouse femur using the Bullet Blender determined with Agilent 2100 Bioanalyzer (the highest number possible is 10). These numbers are sufficient for RT qPCR, microarray and next generation sequencing applications. From Carter LE, Kilroy G, Gimble JM...
What is the difference between plasmid DNA and chromosomal DNA? What are histones? What is chromatin? What is a chromatin? Typically, how many replication origins are formed in: a. prokaryotes? b. eukaryotes? What is DNA sequencing?