2 O, 1 µl of pUC (or the appropriate) primer at a 0.5 pmol/µl stock concentration, and 2 µl of 5x Sequenase buffer. Mix and centrifuge to remove any debri. Heat 2 min, 65℃ and cool slowly to below 35℃ by removing the heat block with samples in it from the heater to...
Be aware that sequencing ITR is challenging, so we request concentrated stocks of DNA. Optimal sequencing concentration for Viral Plasmid is 300 ng/µl (minimum 200 ng/µl). Please submit as much template as you are able, up to 10 µl but no less than 5 µl. Please...
ConcentrationUV absorptionDefault 1 mg/ml†; Specific concentration provided upon request Sequence AccuracyNew!SequencingSequencing verification match the order requirements HomogeneityAgarose gel electrophoresis90%±10% Supercoiled** Restriction AnalysisEnzyme digestion and electrophoresisDNA bands with expected si...
With the advent of genomic sequencing, plasmid DNA is also being sequenced, thus plasmids can now be typed according to their genetic features (Barer, 2018). Pathogenic bacteria are known to possess plasmids that carry genes for various virulence factors enabling them to successfully colonize hosts...
Concentration Nanodrop ✓ ✓ ✓ Identity Plasmid size Restriction digestion ✓ ✓ ✓ Insert sequence Sanger sequencing Optional Optional Optional Full-length plasmid sequence Sanger sequencing Optional Optional Optional NGS Optional Optional Optional Purity OD260/280 Nanodrop ✓ ✓ ✓ Supercoile...
Yields are commonly 1 mg supercoiled plasmid DNA/100 ml culture and the DNA is stable for years if stored in aliquots at –20°C. Injection mixes are assembled from two or more such DNA preparations by mixing in IB to a final concentration of 0.1 mg/ml for each species. Typical nuclear...
The plates were incubated in ambient air at 35 1C for 18 h. The inoculating concentration of 1Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China and 2Anhui Center for Surveillance of Bacterial Resistance, Hefei, Anhui, China 3These ...
Sequence AccuracyNew!SequencingSequencing verification match the order requirements Restriction AnalysisEnzyme digestion and electrophoresisDNA bands with expected sizes detected by agarose gel electrophoresis Note:†The reading of plasmid concentration may varies in the results of multiple measurements, with th...
Concentration > 50 ng/μL Enzyme digestion validation Sanger sequencing Endotoxin-free maxiprep plasmid Pronucleus injection plasmid preparation Service Procedure Confirm gene(s) of interest, design and establish gene editing strategy; Design plasmid sequence and send to the client for confirmation; Constr...
(red, dashed lines). The experiment was conducted for 56 transfers corresponding to about 340 generations in L populations, 450 generations in M populations, and 560 generations in S populations.bSequencing coverage distribution of ancestral and evolved pCON variants from population S6. The presented...