2 O, 1 µl of pUC (or the appropriate) primer at a 0.5 pmol/µl stock concentration, and 2 µl of 5x Sequenase buffer. Mix and centrifuge to remove any debri. Heat 2 min, 65℃ and cool slowly to below 35℃ by removing the heat block with samples in it from the heater to...
ConcentrationUV absorptionDefault 1 mg/ml†; Specific concentration provided upon request Sequence AccuracyNew!SequencingSequencing verification match the order requirements HomogeneityAgarose gel electrophoresis90%±10% Supercoiled** Restriction AnalysisEnzyme digestion and electrophoresisDNA bands with expected si...
Elution in as little as 30 μl provides concentrated DNA for use in downstream applications, such as restriction digests, DNA sequencing, PCR and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging...
Yields are commonly 1 mg supercoiled plasmid DNA/100 ml culture and the DNA is stable for years if stored in aliquots at –20°C. Injection mixes are assembled from two or more such DNA preparations by mixing in IB to a final concentration of 0.1 mg/ml for each species. Typical nuclear...
DNA Clean Up & Concentration PCR Reagents RT-qPCR Reagents Molecular Cloning and Next-Generation-Sequencing Enzymes RNA Isolation DNA and RNA Detection Yeast Research Tools Molecular Biology Accessories, Buffers & Reagents Bioassays Reagents & Chemicals ...
(EB) have been effectively used in curing bacterial plasmids, although the methods can possibly induce mutations in the host chromosomal DNA. The agent's concentration can range from 0.1 to 0.5 mg/ml and depends on the organism and the curing agent used (Letchumanan et al., 2015)....
This could mean that, compared to bacteria, plasmid colonization of the infant gut is less influenced by inheritance from the mother, because the newborn gut environment differs from that of the mother in significant ways (e.g., bacterial density, oxygen concentration, host modulation); this ...
The plates were incubated in ambient air at 35 1C for 18 h. The inoculating concentration of 1Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China and 2Anhui Center for Surveillance of Bacterial Resistance, Hefei, Anhui, China 3These ...
Quick concentration and desalination of plasmid and cosmid DNA eluates obtained by anion-exchange chromatography Typical downstream applications: transfection, in vitro transcription, automated or manual sequencing, cloning, hybridization, and PCR Additional product information Please see the product's Cer...
by 19.5%,1.45-fold and 9.5-fold;indicating that size effect on xylanase expression had an increasing trend from plasmid concentration to xylanase activity.The study demonstrated that plasmid-size was important for over-expression of protein,thus,a small-sized plasmid was better for genetic ...