Plasmid segregation: how to survive as an extra piece of DNA. Crit Rev Biochem Mol Biol 45 - Salje - 2010 () Citation Context ...The stb Operon of Plasmid R388 are though to employ a principle of dynamic oscillation between specific surfaces such as membrane or bacterial chromosome to ...
area of known sequence close to the target). In contemporary usage, purified PCR products most often serve as the sequencing template, but the flexibility of the technique allows for different types of single- or double-stranded DNA to be used (another...
for small-scale projects focusing on one or two genes, while NGS is ideal for higher-throughput sequencing needs. While fragment analysis does not provide sequence information, it can be used to generate relative quantitation information in a cost-effective manner due...
However, unlike the genomic code for proteins, the cis-regulatory code is not a universal code. Cell types use vastly different TF motifs and syntax rules; even a few deviating details can make it impossible to accurately predict which cis-regulatory sequences are read out by a cell type. ...
How do cells use the base sequence of a DNA molecule to produce a polypeptide? Describe how eukaryotic DNA is arranged in the cell. During DNA replication, how are the new nucleotides added to the template strand? Explain about DNA structure. ...
Explain how PCR is used to amplify DNA sequences. Describe three ways that PCR is similar to DNA replication in cells. What is PCR and what does it stand for? How does PCR work? How is a plasmid different from human DNA? What are the differences and similarities between DNA polymerase ...
Molecular cloning, nucleotide sequence, and marker exchange mutagenesis of the exo-poly-alpha-D-galacturonosidase-encoding pehX gene of Erwinia chrysanthem... CUCPB5009 retained a slight ability to pit polygalacturonate semisolid agar and macerated chrysanthemum pith tissues when large numbers of ...
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afunction similarly, we cloned and inserted the complementary DNA (cDNA) fragment containing the 3 ′ -UTR sequence of PRKC ε downstream of firefly luciferase into the pMIRREPORT miRNA expression reporter vector (Applied Biosystems) and then transfected this plasmid into HeLa cells. 正在%[translate...
because I've used this enzyme first hand in past biolabs. The idea of this enzyme is that it can take protein expression from one organism and pull it back into another organism to make it produce the protein desired. This is how Plasmid DNAs also came about, and more important, WHY TH...