How-To Extract Plasmid DNA How To Run the Bolt Tank and Gel How To use the DynaMag™-5 Magnet qPCR How to Label Technical Replicates in Life Technologies Real-Time PCR Software — Ask TaqMan: Ep. 1 Hints for Real-Time PCR (qPCR) Assay Design — Ask TaqMan:...
PCR and sequencing primer design Sanger sequencing targets a region of interest in template DNA using an oligonucleotide sequencing primer, which binds upstream of the target that is to be sequenced (there must be an area of known sequence close to ...
How to start your monocot CRISPR/Cas project: plasmid design, efficiency detection, and offspring analysisCas12aGenome editingPlant transformationPromoterProtoplastSchematic of the T-DNA region in a binary vector for genome editing in monocots. : promoter. : promoter. : maize promoter and the first ...
Primer Design for PCR Primers are always specified 5′ to 3′, left to right. Verify that your primers are designed and ordered in the correct orientation. Primers for PCR and sequencing should be between 18 to 25 nucleotides in length. Primers for PCR and sequencing should have a GC co...
to generate a double strandbreak (DSB) in the DNA at a specific locus in the genome. To achieve thisprecise DSB researchers have developed engineered nucleases, also termed “molecularscissors”. Previous efforts have focused in the molecular understanding andredesign of different protein templates, ...
Alternatively, a CRISPR–Cas9-based approach was used55, and strains were transformed using a plasmid expressing Cas9, a guide RNA and double-stranded PCR repair template from the same plasmids, followed by removal of the Cas9 plasmid by 5-fluoroorotic acid selection. PP7 loops were introduced ...
This can be used to our advantage, for example localization signals can be added to exogenous proteins (usually encoded in a plasmid) so their localisation is predetermined. Adding a nuclear localisation signal (NLS) to virtually any protein would result in protein re-routing to the ...
In addition to safer pharmaceutical properties, such as no risk of genome integration, the transient expression of mRNA-encoded antibodies enables a more controlled exposure, with more protein production during peak expression compared to naked pDNA [17]. Because additional sequences such as plasmid ...
Look for culture systems that set you up for success from the very beginning, enabling you to work from a homogenous starting population. CRISPR/Cas9 for accurate gene editing Delivery of CRISPR/Cas9 for gene editing can be done via plasmid or viral-based methods; however, these pro...
Plasmids (plasmid DNA) How multimodal, flexible suites and facilities work Multimodal, flexible suites and facilities can deliver on these requirements. Multimodal, flexible suites comprise a single operating suite or room that is designed to accommodate the production of a number of modalities – defi...