How-To Extract Plasmid DNA How To Run the Bolt Tank and Gel How To use the DynaMag™-5 Magnet qPCR How to Label Technical Replicates in Life Technologies Real-Time PCR Software — Ask TaqMan: Ep. 1 Hints for Real-Time PCR (qPCR) Assay Design — Ask TaqMan:...
PCR and sequencing primer design Sanger sequencing targets a region of interest in template DNA using an oligonucleotide sequencing primer, which binds upstream of the target that is to be sequenced (there must be an area of known sequence close to ...
whereby a simple exchange of the RNA guide sequence can be employed to re-engineer nuclease specificity, leading to a revolution in the life sciences. Cas9, belonging to Class 2 Type II CRISPR-Cas interference system, is the more extensively used tool for genome...
Alternatively, a CRISPR–Cas9-based approach was used55, and strains were transformed using a plasmid expressing Cas9, a guide RNA and double-stranded PCR repair template from the same plasmids, followed by removal of the Cas9 plasmid by 5-fluoroorotic acid selection. PP7 loops were introduced ...
Approximately 104copies of target DNA are required to detect product in 25-30 PCR cycles Use 1pg–1ng ofplasmid or viral templates Use 1ng–1µg of genomic templates Higher DNA concentrations decrease amplicon specificity (i.e., extra bands are more likely), particularly when a large number ...
Finally, a quality control strategy is developed, which is a set of monitoring processes and final product tests, to ensure the manufacturing process is followed and documented. In conclusion, the design and manufacturing of a human challenge agent is a stepwise process, from establishing a TPP,...
However, colonies made up of cells that accept a plasmid with the DNA insertion remain white, because the DNA that was inserted into the cloning vector at the MCS disrupts the lacZ gene making it impossible for the cell to create a functional enzyme to metabolize x-gal. In other words, ...
Plasmids (plasmid DNA) How multimodal, flexible suites and facilities work Multimodal, flexible suites and facilities can deliver on these requirements. Multimodal, flexible suites comprise a single operating suite or room that is designed to accommodate the production of a number of modalities – defi...
The worldwide emergence of antibiotic resistance poses a serious threat to human health. A molecular understanding of resistance strategies employed by bacteria is obligatory to generate less-susceptible antibiotics. Albicidin is a highly potent antibact
Look for culture systems that set you up for success from the very beginning, enabling you to work from a homogenous starting population. CRISPR/Cas9 for accurate gene editing Delivery of CRISPR/Cas9 for gene editing can be done via plasmid or viral-based methods; however, these pro...