Plasmids are used extensively in basic and applied biology. However, design and construction of plasmids, specifically the ones carrying complex genetic information, remains one of the most time-consuming, labor-intensive, and rate-limiting steps in performing sophisticated biological experiments. Here, ...
Conclusions: The method described here is a fast and easy way to make suitable plasmid out of commercially available plasmids. This process can considerably decrease the time and cost of plasmid construction. Availability of suitable restriction sites in proper order makes it possible to directionally...
The GeneArt™ gene synthesis platform can design plasmid vectors from modular, easily exchangeable fragments encoding regulatory and functional elements or from custom-made elements. These individual “modules” can be obtained via PCR amplification in some cases, or they can be ...
DNAs are high molecular-weight molecules with double strands that contain transgenes for specific proteins. In therapeutics, DNA inhibits the generation of specific protein through the release of transgenes into cells. The design and construction of plasmids with transgene(s) of interest is an impor...
PlasmidMaker is a versatile, automated, and high throughput end-to-end platform for plasmid construction 论文摘要 质粒广泛用于基础和应用生物学。然而,质粒的设计和构建,特别是那些携带复杂遗传信息的质粒,仍然是进行复杂生物实验中最耗时、劳动密集和限速的步骤之一。作者构建了了名为 PlasmidMaker 的多功能、强...
Fast—custom gene synthesis and sub-cloning in as little as 2 weeks Easy—just send us your sequence, we’ll take care of synthesis and cloning Here are two examples of recently completed Syn2Clone projects: Design, synthesis, and cloning of a complex miR precursor in 2 weeks Design, synth...
Applicable quality control data, based on selected scale and options, provided on the standard Certificate of Analysis (CofA). Further documentation available upon request: Material documentation—Documentation of gene construction including gene design, cloning strategy...
From custom vector design to high-throughput protein expression, we have a solution to meet your needs. Plasmid Services GeneArt® Plasmid Services include flexible offerings covering construction of plasmid vectors customized for your needs, and subc...
Plasmid Preparation Unlock the potential of plasmid DNA across multiple therapeutic approaches, from gene and cell therapies, viral vector production, and immunotherapeutics to mRNA/DNA vaccines and nucleic acid drugs. PackGene provides plasmid preparation services that are ideal for any phase of the ...
essentially any DNA sequence encoding the polypeptide of interest can be used. Those skilled in the art will recognize, however, that in practice it is often advantageous to design donor sequences to include or exclude certain codons or sequences. For example, a donor sequence can be designed ...