Another drawback is the requirement of sequence information of the DNA to design primers. Additionally, the primers may occasionally anneal to the wrong sites of DNA. Such non-specific annealing of the primers may result in the amplification of the wrong fragment of DNA. In a rare scenario,...
This pandemic-inspired mRNA gold rush has raised challenges, however, boosting the demand for new, integrated technologies that can improve research, development and manufacturing. For example, we know how to deliver mRNA to some tissues of the body, the liver primarily, but researchers a...
All expression plasmids are available from T.M. under a material transfer agreement with St Jude Children’s Hospital. Code availability Code needed to reproduce the results, SAXS data, CD data, experimental binodals, pH-dependent measurements, sequences used in the bioinformatics analysis and ...
Nanotechnology could help the fight against COVID-19 through different approaches, such as avoiding viral contamination and spray by: (a) design of infection-safe personal protective equipment (PPE) to enhance the safety of healthcare workers and development of effective antiviral disinfectants and ...
Plasmids (plasmid DNA) offer the promise to be used as a drug product. However, its most common use is still as a critical raw material for the manufacturing of viral vectors and to support cell therapy programmes. It has become an essential critical raw material within the realm of newer ...
Yeast strains and plasmids All strains were derived from BY4741 and BY4742 anchor-away background strains54. FRB-tags for anchor-away were introduced either by transformation with a polymerase chain reaction (PCR) product containing the FRB-yEGFP1-hphMX4 cassette (pTL100) or the FRB-mScarlet-...
aI’d rather stay quiet than explain my problems to those who don’t care about me 我宁可会停留沉寂比解释我的问题对对我不关心的那些人[translate] aTo construct the truncated PRE plasmids, different PRE fragments were amplified by PCR and inserted into the NotI and XbaI sites of the cG const...
plasmids that were unstable in other strains.Recent achievements in chemical synthesis technology for large DNA segments together with the rapid development of the wholegenome sequencing,have transferred synthesis of genes to assembly of the whole genomes based on oligonucleotides,and thus created strong ...
One such approach is cyclization, connecting the enzyme's N-terminus to the C-terminus. Cyclization can be accomplished via split inteins4–7, sortases8, carbodiimide cross-linking9 and, most recently, via the design of SpyRings10. SpyTag is a peptide tag that we engineered from Streptococcus...
Here we show that in adapting to treatment with a nAb that suppresses F activation, the complex evolves to become more readily triggered while evading nAb binding. In order to design nAbs with a higher barrier to resistance, that would be therefore more useful clinically, it is necessary to ...