Here, the optimization of a gene transfer protocol for renal cell carcinoma using lipofection as a nonviral gene transduction system was evaluated. To select the promoter which gives the highest expression, dif
Explain how plasmids are used in gene cloning. Describe how to construct recombinant DNA molecules. (a) What are the different types of DNA repair? (b) How do they repair the damage? How does the structure of DNA fit its function, especially as it relates to DNA replication?
Transcriptional bursting has been linked to the stochastic positioning of nucleosomes. However, how bursting is regulated by the remodeling of promoter nucleosomes is unknown. Here, we use single-molecule live-cell imaging of GAL10 transcription in Sacch
Next, we assessed protein expression for each delivery vehicle, as the relationship between nanoparticle biodistribution and protein expression does not always correlate. To do this, we formulated nanoparticles with unmodified firefly luciferase-encoding mRNA (mLuc) and delivered them intravenously at a ...
plasmids that reach the nucleus. Nuclear uptake of plasmid DNA further requires either an increase of nuclear permeability or an active nuclear transport via the nuclear pore. A better understanding of the cellular and molecular bases of the physical gene-transfer process may provide strategies to ov...
Influenza virus can escape most antibodies with single mutations. However, rare antibodies broadly neutralize many viral strains. It is unclear how easily influenza virus might escape such antibodies if there was strong pressure to do so. Here, we map al
Finally we also used plasmids for the construction of standard curves. This allowed us to calculate gene-specific PCR efficiencies for every gene to assure an accurate quantification. The validity of the protocol was demonstrated by means of the study of six genes of the flavonoid biosynthesis ...
Finally we also used plasmids for the construction of standard curves. This allowed us to calculate gene-specific PCR efficiencies for every gene to assure an accurate quantification. The validity of the protocol was demonstrated by means of the study of six genes of the flavonoid biosynthesis ...
Genomic DNA or plasmids containing the gene of interest can be used as standard for validation of assays for DNA analysis, preferably after excising a fragment containing the target sequence to remove interfering supercoiling [26]. Still another option is to use synthetic templates (e.g. gBlocks...
Finally, glycine and serine residues act as non-equivalent spacers, and thus make the glycine versus serine contents an important determinant of the driving forces for phase separation. The totality of our results leads to a set of rules that enable comparative estimates of composition-specific ...