Finally, glycine and serine residues act as non-equivalent spacers, and thus make the glycine versus serine contents an important determinant of the driving forces for phase separation. The totality of our results leads to a set of rules that enable comparative estimates of composition-specific ...
Transcriptional bursting has been linked to the stochastic positioning of nucleosomes. However, how bursting is regulated by the remodeling of promoter nucleosomes is unknown. Here, we use single-molecule live-cell imaging of GAL10 transcription in Sacch
Bacterial colonies with an empty plasmid (i.e., plasmids without a DNA insert) turn blue. This occurs because of a process called alpha-complementation in which the lacZ gene in the cloning vector complements the lacZ deficient E.coli cells. The gene expression of lacZ is induced by IPTG an...
Physical methods represent a promising approach for the safe delivery of therapeutic plasmid DNA in genetic and acquired human diseases. However, their development in clinics is limited by their low efficacy. At the cellular level, efficient gene transfer is dependent on several factors including extra...
Incidents of viral outbreaks have increased at an alarming rate over the past decades. The most recent human coronavirus known as COVID-19 (SARS-CoV-2) has already spread around the world and shown R0 values from 2.2 to 2.68. However, the ratio between m
Two small, multicopy, expression plasmids were constructed that permit convenient insertion of trpR, the structural gene for the trp repressor of Escherich... JL Paluh,C Yanofsky - 《Nucleic Acids Research》 被引量: 171发表: 1986年 Microsatellite instability: an update Deficient DNA mismatch repai...
aI’d rather stay quiet than explain my problems to those who don’t care about me 我宁可会停留沉寂比解释我的问题对对我不关心的那些人[translate] aTo construct the truncated PRE plasmids, different PRE fragments were amplified by PCR and inserted into the NotI and XbaI sites of the cG const...
Here, the optimization of a gene transfer protocol for renal cell carcinoma using lipofection as a nonviral gene transduction system was evaluated. To select the promoter which gives the highest expression, different plasmids which are able to express Escherichia coli beta-galactosidase gene as a ...
(PDB ID: 6FM1). However, it remained still largely unknown how the phage S-2L incorporates the base Z in its genome, especially as no gene corresponding to a DNA polymerase could be detected. This is in contrast with the situation in the phage φVC8, where a DNA polymerase of the ...
One such approach is cyclization, connecting the enzyme's N-terminus to the C-terminus. Cyclization can be accomplished via split inteins4–7, sortases8, carbodiimide cross-linking9 and, most recently, via the design of SpyRings10. SpyTag is a peptide tag that we engineered from Streptococcus...