Incidents of viral outbreaks have increased at an alarming rate over the past decades. The most recent human coronavirus known as COVID-19 (SARS-CoV-2) has already spread around the world and shown R0 values from 2.2 to 2.68. However, the ratio between m
Paramyxoviruses including measles, Nipah, and parainfluenza viruses are public health threats with pandemic potential. Human parainfluenza virus type 3 (HPIV3) is a leading cause of illness in pediatric, older, and immunocompromised populations. There ar
Parsing the genomic sequence of cyanophage S-2L (AX955019) in the search for a protein involved in DNA replication, we identified one ORF corresponding to a member of the Archaeo-Eukaryotic Primase (AEP) superfamily, which had not been noted earlier. We will refer to it as “PrimPol”, ...
For variable culture volumes and further centrifugation steps additional equip- ment is needed. In this Application Note, the production of a recombinant protein is presented using a workflow for culturing and har- vesting bacteria and purifying proteins. The aim is to demonstrate that a high yield...
Resulting RNA-protein complexes were resolved on a native 7% acrylamide gel for 8.5 h at 4°C. Gels were dried and exposed to X-ray film for approximately 8 h at −80°C in the presence of an intensifying screen. Bioinformatic Analyses of U8 Secondary Structures Determination of the ...
Human airway epithelial (HAE) cellular models are widely used in applicative studies of the airway physiology and disease. In vitro expanded and differentiated primary HAE cells collected from patients seem to be an accurate model of human airway, offeri
Human airway epithelial (HAE) cellular models are widely used in applicative studies of the airway physiology and disease. In vitro expanded and differentiated primary HAE cells collected from patients seem to be an accurate model of human airway, offeri
filamentous M13-like phages [27,32]. The most widely studied sites for phage integration are known as duplicated repeat sequence 3 (dRS3) [26], which belong to the family of Neisserial intergenic mosaic elements (NIMEs). Plasmids, such as pJS-A and pJS-B, also play an important role [...
Towards a generic route to thermo-resilience, we established the SpyRing approach, cyclizing enzymes by sandwiching between SpyTag and SpyCatcher (peptide and protein partners which lock together via a spontaneous isopeptide bond). Here we first investigated the basis for this resilience, comparing ...
It would be interesting to establish whether the intervention of these entry pathways may be useful in the design of antiviral drugs that can be used in dengue severe disease. Methods Cells and viruses Murine macrophage P388D1 cells were kindly provided by Dr. Michael Diamond (Washington ...