Display enzyme sites, features, primers, ORFs, translations and more on plasmid maps or in detail on the sequence view Annotate features on your sequences using SnapGene’s curated feature database or your own custom features Add your vector directly into a cloning simulation with all restriction...
Plasmid maps and sequences will always include an ori which is short for theorigin of plasmid replication, and if you are looking at a linear plasmid sequence, it will be at the start. Whatever you do, don’t change it! Once a plasmid is unable to replicate, it is useless. Another th...
Scripts, plasmid maps, and amplicon maps for: Multiplex profiling of developmental enhancers with quantitative, single-cell expression reporters - shendurelab/scQers
View plasmid maps and sequence trace files Annotate features on your plasmids Share your sequences with colleagues Sequence editing and alignment Simulate cloning, PCR and mutagenesis Customize annotations and enzyme sets Automatically create graphical history...
21.1A) or (2) cloning sequences downstream of a gene of interest into the multiple cloning site in p6LacO128 and digesting the resulting plasmid with a restriction enzyme that cleaves within these sequences to direct integration of the LacO array and URA3 at that locus (Fig. 21.1C). The...
Plasmid maps are graphical representation of plasmids that show the locations and relative positions of major elements on the sequence, such as the origin of replication, genes of interest, restriction enzyme sites, and primer binding sites, in addition to the plasmid name and length. Plasmid maps...
aSchematic of the plasmid copy number calibration construct. PhlF-RFP is expressed from a second plasmid (pSB235-237). “Ori” indicates where the plasmid origin is changed in the plasmid being measured. Plasmid maps and part sequences are provided in Supplementary Fig.S18and TableS3.bImages...
Restriction maps and sequences for (1), (4) and (6) are reproduced. Independent claims are also included for the following: (a) composite plasmid (CP1) for autonomic replication in corynefrom bacteria containing at least part of the nucleic acid sequences of these plasmids; at least one ...
PlasmidID is acomputational pipelineimplemented inBASHthat maps Illumina reads over plasmid database sequences. The k-mer filtered, most covered sequences are clustered by identity to avoid redundancy and the longest are used as scaffold for plasmid reconstruction. Reads are assembled and annotated by...
and studies aiming to increased cellulose productivity. Here we present a high-quality draft genome sequence forG. hanseniiATCC 53582 and find that in addition to the previously described cellulose synthase operon, ATCC 53582 contains two additional cellulose synthase operons and several previously ...