Gain Unparalleled Visibility of Your Sequences Open sequence files in SnapGene to view features, annotate, clone and use as a reference Display enzyme sites, features, primers, ORFs, translations and more on plasmid maps or in detail on the sequence view Annotate features on your sequences using...
Plasmid maps and sequences will always include an ori which is short for the origin of plasmid replication, and if you are looking at a linear plasmid sequence, it will be at the start. Whatever you do, don’t change it! Once a plasmid is unable to replicate, it is useless. Another ...
View plasmid maps and sequence trace files Annotate features on your plasmids Share your sequences with colleagues Sequence editing and alignment Simulate cloning, PCR and mutagenesis Customize annotations and enzyme sets Automatically create graphical history...
Plasmid maps are graphical representation of plasmids that show the locations and relative positions of major elements on the sequence, such as the origin of replication, genes of interest, restriction enzyme sites, and primer binding sites, in addition to the plasmid name and length. Plasmid maps...
Plasmid maps and part sequences are provided in Supplementary Fig. S18 and Table S3. k Dynamics of transcript copy number from the constitutive promoter PJ23101 after rifampicin addition. The data was fitted to a single exponential decay. The resulting degradation half-life (μ) is 6.8 ±...
Scripts, plasmid maps, and amplicon maps for: Multiplex profiling of developmental enhancers with quantitative, single-cell expression reporters Note: scripts contain herein do not currently constitute streamlined pipelines, and are shared for transparency. Iterations and improvement will be made in near ...
21.1A) or (2) cloning sequences downstream of a gene of interest into the multiple cloning site in p6LacO128 and digesting the resulting plasmid with a restriction enzyme that cleaves within these sequences to direct integration of the LacO array and URA3 at that locus (Fig. 21.1C). The...
Molecular tools for working with plasmids are well-developed, and include many restriction enzymes which cut the plasmid ring wherever the specific known DNA sequence characteristic of the enzyme appears.1 Plasmid diagrams showing the relative locations of these sequences are known as restriction maps....
Nucleic acid sequences and plasmid maps are provided in supporting datasets of the paper. Source data for figures and original gel images are provided with this paper. References Koch, A. & Kogel, K. H. New wind in the sails: improving the agronomic value of crop plants through RNAi-...
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