Using a plasmid reporter assay, TFs and co-factors are tethered to various inactive enhancers to test their ability to restore enhancer activity. This shows that a wide variety of factors are activating or repressing. They sometimes function in a context-dependent manner, showing that TFs and co...
Why do we extract RNA and then convert it into cDNA to put in the PCR instead of just extracting DNA? What is the function of the deoxynucleotide triphosphates in a PCR reaction? How is plasmid DNA purified from RNA and genomic DNA?
antiparallel and complementary in nucleotide sequence to the template strand of the DNA. The other strand of DNA, referred to as either the nontemplate strand or the coding strand, has the same 5' to 3' orientation and sequence as the mRNA except that T ...
-processingof its own crRNA has been used to modify multiple genetic elementssimultaneously generating constitutive, conditional, inducible, orthogonal andmultiplexed genome engineering of endogenous targets using multiple CRISPR RNAsdelivered on a single plasmid . The viability of this approach has been fur...
The restriction endonuclease and modification methylase genes lie adjacent to each other on the host chromosomal or plasmid DNA and may be oriented transcriptionally in a convergent, divergent, or sequential manner. Occasionally, in convergent or divergent gene organization, an open reading frame ...
S1A). Corresponding results defining the contact mapping on the dystrophin ABD2 fragment characterize several peptides involved in the interaction with F-actin. Interestingly all are included in repeats R11, R12 or R15. These fragments are delimited by Phe1461 to Arg1470, Ser1517 to Arg1527, ...
Influenza virus can escape most antibodies with single mutations. However, rare antibodies broadly neutralize many viral strains. It is unclear how easily influenza virus might escape such antibodies if there was strong pressure to do so. Here, we map al
[22]. In addition to becoming one of the most commonly used dominant selectable markers in fungal transformation, this work pioneered plasmid mediated cassette integration in filamentous fungal genomes using the vector pAN7-1. Moreover, the authors further validated the use of theA. nidulans...
Using a 2-plasmid replication reporter system [75,76], VCP inhibition had no effect on RNA template or viral non-structural protein (nsP) levels, but decreased RNA replication products were observed. Confocal microscopy revealed co-localization of nsPs with VCP [33]. These nsPs are required ...
The restriction endonuclease and modification methylase genes lie adjacent to each other on the host chromosomal or plasmid DNA and may be oriented transcriptionally in a convergent, divergent, or sequential manner. Occasionally, in convergent or divergent gene organization, an open reading frame ...