mg supercoiled plasmid DNA/100 ml culture and the DNA is stable for years if stored in aliquots at –20°C. Injection mixes are assembled from two or more such DNA preparations by mixing in IB to a final concentration of 0.1 mg/ml for each species. Typical nuclear injection volumes are ...
it is a labor intensive and time-consuming process. Excess vortexing of DNA-lipid complexes or DNA solutions may result in some shearing, especially with larger molecules, thereby reducing transfection efficiency. In addition, the concentration of EDTA in the diluted DNA should ...
The optimal ratio of transfection reagent to DNA is highly cell type-dependent. As a starting point, the amount of transfection reagent should be varied while keeping a constant plasmid DNA concentration (for example, 1:1, 3:1, and 5:1 ratios of volume to mass). Additi...
With the increase of DNA concentration and increasing N/P ratio, transfection efficiency also increased. Our results showed a better transfection efficiency with the circular compared to the linearized DNA, under the same experimental conditions for both Lipofectamine and PEI. In electron microscopy, ...
Saravolac EG, Ludkovski O, Skirrow R et al. Encapsulation of plasmid DNA in stabilized plasmid-lipid particles composed of different cat- ionic lipid concentration for optimal transfection activity. J Drug Tar- get 2000; 7: 423-37.E.G. Saravolac, et al., Encapsulation of plasmid DNA in ...
Cells were seeded on 35-mm glass-bottom culture dishes (MatTek, MA, USA) at a concentration of 2 × 105per dish one day before imaging. The cells were incubated for 4 h with Effectene transfection reagent (Qiagen) containing 0.5 μg/dish DNA plasmid. Hoechst 33342 was added to a...
the transfected efficiency of C1-ORF2,C1-ORF2as,and C1-280-1×14 as firstly increased then decreased or kept fair with the transfected amount increased.Conclusion The type and amount of transfected plasmids and the concentration of transfected cells influenced the expression of transfected plasmids....
Note:†The reading of plasmid concentration may varies in the results of multiple measurements, with the approximate range from -5~+15%. * Sequence verification of full gene insert only applies when bundled with gene synthesis (an insert to be cloned into a plasmid). ...
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Quick concentration and desalination of plasmid and cosmid DNA eluates obtained by anion-exchange chromatography Typical downstream applications: transfection, in vitro transcription, automated or manual sequencing, cloning, hybridization, and PCR Additional product information Please see the product's Cer...