Yield and concentration for plasmid DNA isolated using the ZymoPURE II Maxiprep kit compared to two endotoxin-free plasmid purification kits from Supplier Q and Supplier MN.Plasmid DNA (pGL3®) was isolated from 150 ml of JM109E. coliculture grown overnight following the manufacturer’s suggested...
Plasmid acquisition is an important mechanism of rapid adaptation and niche expansion in prokaryotes. Positive selection for plasmid-coded functions is a major driver of plasmid evolution, while plasmids that do not confer a selective advantage are consi
a strain constitutively expressing mScarI, thus mScarI production is reported as a relative (“Rel.”) value.aThe AHL-LuxR transcription inducible circuit was cloned into TULIP. Expression of sfGFP and
Quick concentration and desalination of plasmid and cosmid DNA eluates obtained by anion-exchange chromatography Typical downstream applications: transfection, in vitro transcription, automated or manual sequencing, cloning, hybridization, and PCR Additional product information Please see the product's Cer...
we can displace the plasmid DNA by washing it with a high concentration of Cl-. The elution solution contains a buffer (at pH8.5, up from pH7.0 in the previous wash step), NaCl at a concentration of 1.25 to 1.60M (considered a high-salt concentration, and up from 1.0M in the previou...
Copy numbers are regulated by inhibition of RNA 11 by the antisense RNA 1, whose concentration is proportional to the plasmid concentration. This inhibition is enhanced by a protein, Rom, and takes place during a time set by the transcription of 250 bases of the gene for RNA 11. When this...
(EB) have been effectively used in curing bacterial plasmids, although the methods can possibly induce mutations in the host chromosomal DNA. The agent's concentration can range from 0.1 to 0.5 mg/ml and depends on the organism and the curing agent used (Letchumanan et al., 2015)....
Copy numbers are regulated by inhibition of RNA 11 by the antisense RNA 1, whose concentration is proportional to the plasmid concentration. This inhibition is enhanced by a protein, Rom, and takes place during a time set by the transcription of 250 bases of the gene for RNA 11. When this...
DNA size marker with fragments of 25, 50, 75, 100, 150, 200, 250, 300, 400, and 500 bp; concentration 100 ng/ul US$105.00 Add to Cart CAT NO./ID 13114-325 Ceramic Beads, 2.8 mm (325 g) Customize your bead tubes with bulk beads for rapid and reliable biological sample lysis fro...
concentration, and therefore each strain can be described by two structurally identifiable parameters53: the resource conversion rate (ρ) and the specific affinity for the resource (Vmax/Km). These parameters were estimated from the ODs of each strain growing in monoculture (with and without ...