The IncI1-I(α) plasmid was harbored in a S. Kentucky serovar (Table 1). According to SPIFinder analysis, we identified 11 different Salmonella Pathogenicity Islands (SPIs) among studied isolates. All isolates including Kentucky and Enteritidis serovars contained SPI-1, SPI-2, SPI-3, SPI-...
accessed on 2 July 2024) were used to extract information about MLST sequence types, acquired antimicrobial resistance genes and chromosomal mutations associated with antimicrobial resistance (ResFinder v4.5.0 [26]),Salmonellapathogenicity island (SPIFinder v2.0,) [27] and plasmid replicon...
Log2 fold-change values were calculated from the sequencing counts between two sets of samples. The specific comparison sets made for each screen are described in section “Overview of cell collection and experimental replicates.” Note that each cell migration assay resulted in two log2 fold-chang...
The discovery of the presence of plasmid pOF39 begs the question: when and how was it introduced? One possibility is that the plasmid was introduced into the lineage between the BM16 and BM25 isolates, that were evolved at 45 °C and 48 °C, respectively. This ideas is consistent with th...
ARTICLE Received 1 Aug 2014 | Accepted 20 Feb 2015 | Published 15 Apr 2015 DOI: 10.1038/ncomms7687 Whole-exome sequencing implicates UBE3D in age-related macular degeneration in East Asian populations Lv-Zhen Huang1,2,3,*, Ying-Jie Li4,*, Xue-Feng Xie5,*, Jing-Jing Zhang1,2,3,*,...
Point mutant clones were submitted to target DNA sequencing (Sanger) and the single nucleotide change was confirmed (data not shown). One point mutant of GyrA D94G was used to perform the MIC determination of QOA derivatives. As shown in Table 4, the MIC for MOX and OFL increased for ...
coli TOP10 F’ cells and the sequences were confirmed by Sanger sequencing (LGC Genomics). Finally, electrocompetent E. coli BL21 (DE3) Gold cells were transformed with plasmid DNA to give nFMO2 expression strains. The nFMO2 expression strains were cultivated as follows: overnight cultures [...
The genotyping results of both the amplified and genomic DNA samples were also compared with reference genotypes of the same samples obtained by direct sequencing. The genomic DNA samples showed genotype concordance rates of 100% for all three dilutions while the concordance rates of the amplified ...
This study has focused on the whole genome sequencing of S. maltophilia in order to identify the genes which are responsible for antibiotic resistance. Initially, 25 samples were taken but as reported by [41] that the whole genome sequencing is a costly process, so, out of the 25 isolates,...
This is because 1) it is easy to map the site of mutation (insertion of the transposon) by sequencing out from the ends of the transposon; 2) transposons can be used which insert only once in the chromosome, allowing analysis of a mutant phenotype resulting from a single insertion mutation...