今天就轻松些,直接上网址。 SGN VIGS Tool:https://vigs.solgenomics.net/ 使用简单,把自己需要沉默的基因cds序列粘贴进去直接RUN即可。SGN VIGS Tool除了可以选取烟草VIGS片段外,该数据库还包含许多物种的基因组。如: 如果你需要的物种或基因组不在列表中,或可以写邮件告知网站的维护人员,说不定就会被添加其中。...
Install the vigs_tool docker To install the vigs_tool system, clone the git repository that contains the example docker-compose.yml file. git clone https://github.com/solgenomics/VIGS Run the vigs_tool from within the VIGS folder using: docker compose up -d By default, the VIGS interfac...
2015. The SGN VIGS Tool: User-friendly software to design virus-induced gene silencing (VIGS) constructs for functional genomics. Mol. Plant 8:486-488.Fernandez-Pozo N, Rosli HG, Martin GB, Mueller LA. The SGN VIGS Tool: User-Friendly Software to Design Virus-Induced Gene Silencing (V...
Figure 1. The SGN VIGS Tool. (A) VIGS tool algorithm linear workflow. Arrows leaving from “Print results” box back to previous steps represent options to recalculate results with different parameters changed by the user. (B) Control panel to recalculate the results with new parameters. (C)...
the only dependencies for the SGN VIGS tool. BLAST is used together with the BioPerl module Bio::BLAST::Database to get the functional descriptions, and sequences from query genes when using gene IDs as input. To install the tool independently of the SGN, a stand-alone version was develope...
Virus-induced gene silencing as a tool to study regulation of alkaloid biosynthesis in medicinal plants [J]. Methods Mol Biol, 2022, 2469: 155-164. [21] Bomzan D P, Kumar K, Kumar S R, et al. Virus-induced gene silencing for functional genomics of specialized metabolism in medicinal ...
Virus-induced gene silencing as a tool for functional analyses in the emerging model plant Aquilegia (Columbine, Ranunculaceae) [J]. Plant Methods, 2007, 3: 6. [8] Peng RH, Jones DC, Liu F, et al. From sequencing to genome editing for cotton improvement [J]. Trends Biotechnol, 2021, ...
Virus-induced Gene Silencing as a Tool forFaMYB5Gene Functional Studies in Strawberry WANG Ling,TANG Haoru*,WANG Xiaorong,CHEN Qing,JIANG Leiyu,and LIN Yuanxiu College of Horticulture,Sichuan Agricultural University,Chengdu611130,China Online:2017-01-25Published:2017-01-25 ...
VIGS技术在辣椒基因功能研究中的应用进展
Genome and EST sequencing projects generate a wealth of sequence information for agronomically and phylogenetically important plant species of which the majority is difficult to subject to functional genomics. Virus-induced gene silencing (VIGS) provides a powerful tool to enable gene functional analysis ...