该论文综述了DNA和RNA病毒在植物体内诱导VIGS的分子机制,以及VIGS作为基因调控工具在基因功能分析中的各种应用。 由于没了底稿,想了想还是拖一拖,后面有机会再写上面规划的目录。今天就轻松些,直接上网址。 SGN VIGS Tool:https://vigs.solgenomics.net/ 使用简单,把自己需要沉默的基因cds序列粘贴进去直接RUN即可。SG...
Stand Alone version of SGN VIGS Tool Developed on a Catalyst framework To add a VIGS dataset, use the following commands: unzip GCA_003287315.1.zip in emacs, replace the string >lcl| with > , if present (otherwise, fastacmd will not work correctly) Copy the dataset to the correct locatio...
the only dependencies for the SGN VIGS tool. BLAST is used together with the BioPerl module Bio::BLAST::Database to get the functional descriptions, and sequences from query genes when using gene IDs as input. To install the tool independently of the SGN, a stand-alone version was develope...
A. The SGN VIGS tool: user-friendly software to design virus-induced gene silencing (VIGS) constructs for functional genomics. Mol. Plant 8, 486-488 (2015).Fernandez-Pozo N, Rosli HG, Martin GB, Mueller LA. The SGN VIGS Tool: User-Friendly Software to Design Virus-Induced Gene Silencing...
Figure 1. The SGN VIGS Tool. (A) VIGS tool algorithm linear workflow. Arrows leaving from “Print results” box back to previous steps represent options to recalculate results with different parameters changed by the user. (B) Control panel to recalculate the results with new parameters. (C)...
git clone https://github.com/solgenomics/VIGS cd VIGS nano docker-compose.yml 对于docker-compose.yml,修改(关键在于把那个mason文件夹给映射进去) services: vigs_tool: ports: - 8088:8088 volumes: - type: bind source: $HOME/vigs_sequence_files ...
A new tool was recently implemented to improve Virus-Induced Gene Silencing (VIGS) constructs called the SGN VIGS tool. With the growing genomic and phenotypic data in the database, SGN is now advancing to develop new web-based breeding tools and implement the code and database structure for ...
A new tool was recentlyimplemented to improve Virus-Induced Gene Silenc-ing (VIGS) constructs called the SGN VIGS tool.With the growing genomic and phenotypic data inthe database, SGN is now advancing to developnew web-based breeding tools and implement thecode and database structure for other...
A new tool was recently implemented to improve Virus-Induced Gene Silenc- ing (VIGS) constructs called the SGN VIGS tool. With the growing genomic and phenotypic data in the database, SGN is now advancing to develop new web-based breeding tools and implement the code...
The SGN VIGS Tool: User-friendly software to design virus-induced gene silencing (VIGS) constructs for functional genomics. Mol Plant. 2015; 8:486-488. [PubMed: 25667001]Fernandez-Pozo N, Rosli H, Martin G, Mueller L. 2015. The SGN VIGS tool: User-friendly software to design virus-...