SGN VIGS Tool:https://vigs.solgenomics.net/ 使用简单,把自己需要沉默的基因cds序列粘贴进去直接RUN即可。SGN VIGS Tool除了可以选取烟草VIGS片段外,该数据库还包含许多物种的基因组。如: 如果你需要的物种或基因组不在列表中,或可以写邮件告知网站的维护人员,说不定就会被添加其中。邮件地址:sgn-feedback@solgen...
image: breedbase/vigs_tool:v0.01 restart: always 开启docker docker compose up-d#输出[+]Running1/1✔Containervigs_tool Started 添加基因组文件 进入docker,想办法把cds文件扔里面,比如windows打开扔进刚才映射的文件夹$HOME/vigs_sequence_files里 docker exec -it vigs_tool bash #进入后 cd /home/vigs_...
The SGN VIGS Tool: User-Friendly Software to Design Virus-Induced Gene Silencing (VIGS) Constructs for Functional Genomics. Mol Plant. 2015; 8: 486-488. doi: 10.1016/j.molp.2014.11.024 PMID: 25667001The SGN VIGS Tool: User-Friendly Software to Design Virus-Induced Gene Silencing (VIGS)...
(http://vigs.solgenomics.net). TheSGNVIGStoolalgorithmsimulates in silico the VIGS pro- cesses occurring in the plant cell (Figure 1A). In the first stage, the target gene is processed in all possible 21-nucleotide frag- ments (or any size chosen by the user between 18 and 24 nucle...
A new tool was recently implemented to improve Virus-Induced Gene Silencing (VIGS) constructs called the SGN VIGS tool. With the growing genomic and phenotypic data in the database, SGN is now advancing to develop new web-based ... N Fernandezpozo,N Menda,JD Edwards,... - 《Nucleic Acids...
Stand Alone version of SGN VIGS Tool Developed on a Catalyst framework To add a VIGS dataset, use the following commands: unzip GCA_003287315.1.zip in emacs, replace the string >lcl| with > , if present (otherwise, fastacmd will not work correctly) Copy the dataset to the correct locatio...
(2015). The SGN VIGS tool: user-friendly software to design virus- induced gene silencing (VIGS) constructs for functional genomics. Mol. Plant 8: 486-488.N. Fernandez-Pozo, H. G. Rosli, G. B. Martin, L. A. Mueller, The SGN VIGS tool: User- friendly software to design virus-...
To install the tool independently of the SGN, a stand-alone version was developed that can be installed as a web tool running on a Catalyst server (https://github.com/solgenomics/VIGS). Not all plant species are amenable to VIGS, and different species show different silencing efficiencies. ...
A. The SGN VIGS tool: user-friendly software to design virus-induced gene silencing (VIGS) constructs for functional genomics. Mol. Plant 8, 486-488 (2015).Fernandez-Pozo N, Rosli HG, Martin GB, Mueller LA. The SGN VIGS Tool: User-Friendly Software to Design Virus-Induced Gene Silencing...