今天就轻松些,直接上网址。 SGN VIGS Tool:https://vigs.solgenomics.net/ 使用简单,把自己需要沉默的基因cds序列粘贴进去直接RUN即可。SGN VIGS Tool除了可以选取烟草VIGS片段外,该数据库还包含许多物种的基因组。如: 如果你需要的物种或基因组不在列表中,或可以写邮件告知网站的维护人员,说不定就会被添加其中。...
To add a VIGS dataset, use the following commands: unzip GCA_003287315.1.zip in emacs, replace the string >lcl| with > , if present (otherwise, fastacmd will not work correctly) Copy the dataset to the correct location, where the VIGS tool can see it. If using the docker, this targe...
This web tool was developed using Perl, JavaScript, AJAX, and HTML5 canvas to allowa fast and interactive graphical represen- tation of the results. BLAST (Altschul et al., 1990) and Bowtie are the only dependencies for the SGN VIGS tool. BLAST is used together with the BioPerl module ...
Figure 1. The SGN VIGS Tool. (A) VIGS tool algorithm linear workflow. Arrows leaving from “Print results” box back to previous steps represent options to recalculate results with different parameters changed by the user. (B) Control panel to recalculate the results with new parameters. (C)...
拉docker,可能需要使用镜像 参照官方教程 git clone https://github.com/solgenomics/VIGS cd VIGS nano docker-compose.yml 对于docker-compose.yml,修改(关键在于把那个mason文件夹给映射进去) services: vigs_tool: ports: - 8088:8088 volumes: - type: bind ...