今天就轻松些,直接上网址。 SGN VIGS Tool:https://vigs.solgenomics.net/ 使用简单,把自己需要沉默的基因cds序列粘贴进去直接RUN即可。SGN VIGS Tool除了可以选取烟草VIGS片段外,该数据库还包含许多物种的基因组。如: 如果你需要的物种或基因组不在列表中,或可以写邮件告知网站的维护人员,说不定就会被添加其中。...
VIGS Stand Alone version of SGN VIGS Tool Developed on a Catalyst framework To add a VIGS dataset, use the following commands: unzip GCA_003287315.1.zip in emacs, replace the string >lcl| with > , if present (otherwise, fastacmd will not work correctly) Copy the dataset to the correct ...
when using gene IDs as input. To install the tool independently of the SGN, a stand-alone version was developed that can be installed as a web tool running on a Catalyst server (https:// github/solgenomics/VIGS). Not all plant species are amenable to VIGS, and different species ...
The SGN VIGS tool algorithm simulates in silico the VIGS processes occurring in the plant cell (Figure 1A). In the first stage, the target gene is processed in all possible 21-nucleotide fragments (or any size chosen by the user between 18 and 24 nucleotides), to get short sequences equi...
参照官方教程 git clone https://github.com/solgenomics/VIGS cd VIGS nano docker-compose.yml 对于docker-compose.yml,修改(关键在于把那个mason文件夹给映射进去) services: vigs_tool: ports: - 8088:8088 volumes: - type: bind source: $HOME/vigs_sequence_files ...