Ulp1 motif 1 (pink) includes strand 2, a loosely conserved region in the Ulp1-like protease family. Motif 1 Smt3 interactions include side chain to main chain hydrogen bonding, van der Waals (VDW) contacts, and one salt bridge between Ulp1 Arg-438 and Smt3 Glu-94 (Figure 1, Figure 4...
Equally crucial to these processes is the removal of SUMO from its targets by the Ulp1 (HuSENP1/2) family of SUMO proteases. Ulp1 activity is normally spatially restricted, because it is localized to the nuclear periphery via interactions with the nuclear pore. Delocalization of Ulp1 causes DNA...
Here we report that specific mutations in the Saccharomyces cerevisiae Ulp1 SUMO protease, including its coiled-coil (CC) domain, lead to the accumulation of distinct sumoylated proteins in vivo. A prominent ~50-kDa sumoylated protein accumulates in a Ulp1 CC mutant. The protein was identified as...
Mutants lacking the Ulp2 SUMO protease are the most severely affected, and this defect was traced to the mutants' impaired ability to induce transcription of INO1, which encodes the rate-limiting enzyme of inositol biosynthesis. Conversely, inositol starvation induces a striking change in the ...
2015. Pli1(PIAS1) SUMO ligase protected by the nuclear pore-associated SUMO protease Ulp1SENP1/2. J Biol Chem 290: 22678-22685.Nie, M.; Boddy, M.N. Pli1PIAS1 SUMO Ligase Protected by the Nuclear Pore-associated SUMO Protease Ulp1SENP1/2. J. Biol. Chem. 2015, 290, 22678-22685....