Site-1 protease(S1P)是激活独特的膜结合潜伏转录因子的关键蛋白酶,被称为各种生物过程中的关键因子。有研究表明,S1P与骨骼发育有关,S1P突变患者表现出骨骼发育不良。然而,S1P在破骨形成中的作用尚不清楚。该研究表明,S1P通过ATF6/CHOP/LC3和SREBP2/LC3...
近日,浙江大学医学院附属邵逸夫医院骨科赵凤东/陈键/刘军辉团队在JCI insight在线发表了S1P regulates intervertebral disc aging by mediating endoplasmic reticulum - mitochondrial calcium ion homeostasis的研究论文,该研究阐明了S1P在椎间盘...
Site-1 protease (S1P) is a Golgi-located protein that activates unique membrane-bound latent transcription factors, and it plays an indispensable role in endoplasmic reticulum stress, lipid metabolism, inflammatory response and lysosome function. A patient with S1P mutation exhibits severe skeletal ...
(C) Domain structure of the human α/β-subunit precursor that is cleaved by site-1 protease (S1P) into mature and catalytically active α- and β-subunits. The luminal domain of the α-subunit contains two stealth domains, two Notch repeat-like domains, the γ-subunit binding domain, and...
Site-1 protease inhibition is accompanied by a distinctive change in gene expression associated with amino acid, steroid and fatty acid synthesis pathways. These results demonstrate that transcriptional control of metabolic programs necessary for secretory activity can be targeted via site-1 protease ...
Site-1 protease (S1P) ablation in the osterix-lineage in mice drastically reduces bone development and downregulates bone marrow-derived skeletal stem cells. Here we show that these mice also suffer from spina bifida occulta with a characteristic lack of bone fusion in the posterior neural arches...
In sterol-depleted cells, SCAP escorts SREBPs from ER to Golgi, where SREBPs are cleaved by Site-1 protease (S1P). Sterols block this transport and abolish cleavage. Relocating active S1P from Golgi to ER by treating cells with brefeldin A or by fusing the ER retention signal KDEL to S1P...
and subsequently lysed with grown for 2 days to 80% detergent buffer (50 mM Tris–HCl, pH 7.4; 150 mM NaCl; 5 mM EDTA; 10 mM NaF; 10 mM disodium pyrophosphate; 1% Nonidet P-40; 0.5% sodium deoxycholate; 0.1% SDS) in the presence of protease and phosphatase ...
and subsequently lysed with grown for 2 days to 80% detergent buffer (50 mM Tris–HCl, pH 7.4; 150 mM NaCl; 5 mM EDTA; 10 mM NaF; 10 mM disodium pyrophosphate; 1% Nonidet P-40; 0.5% sodium deoxycholate; 0.1% SDS) in the presence of protease and phosphatase ...
A crucial step in the arenavirus life cycle is the proteolytic processing of the viral envelope glycoprotein precursor (GPC) by the cellular proprotein convertase (PC) subtilisin kexin isozyme-1 (SKI-1)/site-1 protease (S1P). Here we conducted a systematic and quantitative analysis of SKI-1/...