Sterol regulatory element-binding protein-1 (SREBP1) is cleaved by site 1 protease (S1P) to release the transcriptionally active amino-terminal domain. PF-429242 is a potent and competitive S1P inhibitor. We here tested its activity in RCC cells. In established and prim...
Site-1 protease (S1P) is a Golgi-located protein that activates unique membrane-bound latent transcription factors, and it plays an indispensable role in endoplasmic reticulum stress, lipid metabolism, inflammatory response and lysosome function. A patient with S1P mutation exhibits severe skeletal ...
Bacteria were then lysed with BugBuster Master Mix supplemented with 20 mM imidazole, 0.4 mM PMSF, and protease inhibitor cocktail (P8849; Sigma, St. Louis, MO) and adjusted to pH 7.8. Insoluble debris was pelleted by centrifugation at 30,000 × g for 30 min and the supernat...
This means that the feature selection combined with feature fusion andclassifier parameter optimization can effectively improve HIV-1 cleavage siteprediction. Moreover, our work can provide useful help for HIV-1 protease inhibitordeveloping in the future....
Concomitant use of OXYCONTIN with a CYP3A4 inhibitor, such as macrolide antibiotics (e.g., erythromycin), azole-antifungal agents (e.g., ketoconazole), and protease inhibitors (e.g., ritonavir), may increase plasma concentrations of oxycodone and prolong opioid adverse reactions, which may cause...
Cell pellets were lysed in lysis buffer containing 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 10 mM DTT, 1 mM EDTA, 1 × Protease inhibitor cocktail, 1 mM PMSF, and 1% (v/v) NP-40. Lysates were centrifuged at 20,000 g for 30 min at 4 °C to separate ...
Protease nexin I (PN1)1 is a member of the serine protease inhibitor (SERPIN) super-gene family that acts as an important physiological inhibitor of thrombin and urokinase (1, 2, 3). The mature form of PN1 is a 43-kDa glycoprotein that is found in several tissues throughout the body ...
viruses Article Suppressive Effects of the Site 1 Protease (S1P) Inhibitor, PF-429242, on Dengue Virus Propagation Leo Uchida 1, Shuzo Urata 2, Gianne Eduard L. Ulanday 1,3, Yuki Takamatsu 1, Jiro Yasuda 2,4, Kouichi Morita 1,4 and Daisuke Hayasaka 1,4,* 1 Department of Virology, ...
2,6-Dimethoxy-1,4-benzoquinone aladdin Cat# D154943 Copper (II) chloride aladdin Cat# C106775 Fluocinolone acetonide aladdin Cat# F129244 Complete Protease inhibitor cocktail Sigma Cat# 000000011697498001 PhosSTOP Sigma Cat# 00000004906845001 DMEM, High Glucose, Pyruvate Thermo Fisher Cat# 11995073 ...
Small-molecule inhibitor of USP7/HAUSP ubiquitin protease stabilizes and activates p53 in cells. Molec. Cancer Therap. 8(8): 2286-95 (2009). Cummins et al., HAUSP is required for p53 destabilization. Cell Cycle 3(6): 689-92 (2004). Dayal et al., Suppression of the deubiquitinating...