The level of loaded protein in each gel lane was monitored by re-blotting with an antibody for �-Tubulin (1:1000; Sigma). Anti-rabbit HRP (Sigma) and anti-mouse HRP (Invitrogen) secondary antibodie...Drosophila Ulp1, a nuclear pore-associated SUMO protease, prevents accumulation of ...
Ub/Ubl deconjugation from protein targets also plays a central role in these systems (15, 47). At least 17 yeast deubiquitinating (DUB) enzymes have been identified, 16 in the ubiquitin protease (UBP) family and 1 in the ubiquitin C-terminal hydrolase (UCH) family. DUB enzymes are cysteinyl...
Posttranslational protein modification by the ubiquitin-like SUMO protein is critical to eukaryotic cell regulation, but much remains unknown regarding its operation and substrates. Here we report that specific mutations in the Saccharomyces cerevisiae Ulp1 SUMO protease, including its coiled-coil (CC) ...
Equally crucial to these processes is the removal of SUMO from its targets by the Ulp1 (HuSENP1/2) family of SUMO proteases. Ulp1 activity is normally spatially restricted, because it is localized to the nuclear periphery via interactions with the nuclear pore. Delocalization of Ulp1 causes DNA...
Posttranslational protein modification by the ubiquitin-like SUMO protein is critical to eukaryotic cell regulation, but much remains unknown regarding its operation and substrates. Here we report that specific mutations in the Saccharomyces cerevisiae Ulp1 SUMO protease, including its coiled-coil (CC) ...
Comparative study of the insoluble and soluble Ulp1 protease constructs as Carrier free and dependent protein immobilizates - ScienceDirectUlp1 proteaseEscherichia coliGFIL8 tagActive inclusion bodiesCellulose-binding module tagOriented immobilizationRegenerated amorphous cellulose...
protein degradationsmall ubiquitin-like modifier (SUMO)sumoylationCovalent modification of the proteome by SUMO is critical for genetic stability and cell growth. Equally crucial to these processes is the removal of SUMO from its targets by the Ulp1 (HuSENP1/2) family of SUMO proteases. Ulp1 ...