我们可以构建一个携带眼睛特异性启动子驱动GAL4表达的转基因线,以及一个携带UAS-Gene X的转基因线。通过交配这两个转基因线,在后代果蝇的眼睛中,GAL4将驱动UAS-Gene X的表达,从而允许我们观察Gene X对眼睛发育的影响。 2. 细胞特异性基因敲除 可以将UAS序列与RNA干扰(RNAi)元件结合,用以在特定细胞中敲低目标基...
Introduction of the yeast UAS/GAL4 system into the fly has made such ectopic expression studies easier and more versatile38. With the UAS/GAL4 system, only the construction of a UAS–cDNA construct that drives the gene of interest is required. A fly line carrying the UAS–cDNA construct can...
Quantitative analysis of the GAL4/UAS system in Drosophila oogenesis. Genesis 44, 66-74.Goentoro, L. A., N. Yakoby, J. Goodhouse, T. Schupbach, and S. Y. Shvartsman, 2006 Quantitative analysis of the GAL4/UAS system in Drosophila oogenesis. Genesis 44: 66-74....
UAS-Gal4 Systemdoi:10.1007/3-540-29623-9_9073Definition UAS-Gal4 system refers to an in vivo system that has been adapted from yeast, and consists of two transgenic lines of Drosophila – a driver line (yeast transcription factor Gal4 is expressed under the control of a specific promoter)...
Synonyms Bipartite GAL4 /UAS expression system Definition The GAL4 /UAS system refers to the use of yeast proteins which interact to induce the expression of genes from any organism in a tissue and temporal-specific manner. Characteristics The GAL4 /UAS System There are many tools available ...
传学实验指导》为参考,尝试将果蝇的GAL4-UAS 系统引入遗传学实验,实现基因的表达。在本科遗 传学实验中引入分子遗传学的理念。 1〃GAL4/UAS系统 GAL4蛋白是酵母中的一类转录因子,它能够 与特定的上游激活序列(UpstreamActiveSe- quences,UAS)结合,并驱动UAS下游基因的表达。
GAL4一UAS系统就是一个异位表达外源基因,从而分析基因功能的好方法,并 且取得了成功。 将人类视网膜疾病一视网膜色素变性RP2基因的cDNA克隆至转基因载体 pUAST中,并利用c-myc构建一个融合表达蛋白,在果蝇晚期胚胎所分离出的 Schneider2细胞系中诱导表达,Western检测表达出一个大约40kb的蛋白。与 ...
A GAL4-UAS system for the worm communityLab Animal is a peer-reviewed journal offering information, ideas, methods, and materials for the animal research professionaldoi:10.1038/laban.1240Dustin M. GrahamLab Animal
利用GAL4-UAS系统在果蝇中分析人类基因功能 型旦鱼叁L!型△S丕煎盘墨蝇史婴煎厶耋基固功能 摘要 人类基因组草图基本完成,大量的新基因只有确定的序列及基因组定位信 息。新的焦点是要阐明这些基因的功能,果蝇对此起重要作用。 本研究利用转基因果蝇和GAL4-UAS系统初步鉴定功能基因。首先建立了 源于11个人类基因...
洗膜液1:Urea6M,O.4%SDS,0.5×SSC 洗膜液2:2×SSC (4) 限制性内切酶: Mannheim公司 果蝇GAL4.UAS系统分析人类基因功能技术的初步建立 DNATaq、PCRbuf、Mgcl2 dNTP等购自Promega公司 T4 DNA连接酶、RnaseA购ft Mannheim公司 Boringer 主要仪器与设备’ ELMER公司1 PCR仪:PCR扩增仪GeneAmpPCRsystem2400(美国...