我们可以构建一个携带眼睛特异性启动子驱动GAL4表达的转基因线,以及一个携带UAS-Gene X的转基因线。通过交配这两个转基因线,在后代果蝇的眼睛中,GAL4将驱动UAS-Gene X的表达,从而允许我们观察Gene X对眼睛发育的影响。 2. 细胞特异性基因敲除 可以将UAS序列与RNA干扰(RNAi)元件结合,用以在特定细胞中敲低目标基...
UAS-Gal4 Systemdoi:10.1007/3-540-29623-9_9073UAS-Gal4 system refers to an in vivo system that has been adapted from yeast, and consists of two transgenic lines of Drosophila– a driver line (yeast transcription factor Gal4 is expressed under...Springer Berlin Heidelberg...
Synonyms Bipartite GAL4 /UAS expression system Definition The GAL4 /UAS system refers to the use of yeast proteins which interact to induce the expression of genes from any organism in a tissue and temporal-specific manner. Characteristics The GAL4 /UAS System There are many tools available ...
本文综述了GAL4/UAS系统的建立及其研究应用。关键诃:GAIA/UAS系统;转基因;基因表达ee图分类号:0口89文献标识码:A文章编号:1004—31lX(2006)01—0078—04StudyofGMM/UASSysteminTr剐asgemcTedmologyLIChun—fen91,XIAQi峭一youl,ZHOUZe—yangIo+(1TneKeySefieulturalIabor习l唧0ftheMinimy0fAgfieultu弛,‰...
90年代所涌现的GAL4一UAS系统就是一个异位表达外源基因,从而分析基因功能的好方法,并且取得了成功。将人类视网膜疾病一视网膜色素变性RP2基因的cDNA克隆至转基因载体pUAST中,并利用c-myc构建一个融合表达蛋白,在果蝇晚期胚胎所分离出的Schneider2细胞系中诱导表达,Western检测表达出一个大约40kb的蛋白。与在大肠杆菌中...
effectively.By using germline transformation together with GAL4一UAS system,we studied the possibility of expressing and functionally characterization of human genes in Drosophila.Fifty-four transgenic fly lines corresponding to 10 human genes have been established.When expressed individually by crossing to...
One widely used binary expression system is the GAL4/UAS system, which consists of a driver construct, where expression of the heterologous transactivator GAL4 is driven by an inducible or tissue specific enhancer. In the responder construct, the gene of interest is under the control of the ...
本文 综述了GAL4/UAS系统的建立及其研究应用。 关键词:GAAl/UAS系统 ;转基困;基因表达 中图分类号:(y789 文献标识码:A 文章编号:1004—31Ix(2x【)6)0J 13078一o4 StudyofGAIA /UASSystem inTransgenicTechnology LIChun—feng.XIAQing—you.ZHOUZe—yang (I,TheKeySericulturalLaboratoryoftheMinistryofAb~i...
The Gal4-UAS system has been a go-to tool for researchers working withDrosophila melanogasterfor nearly thirty years. GAL4, a transcription factor originating in yeast, will pair with an upstream activation sequence, or UAS, to manipulate the expression of a gene of interest anywhere in the fl...
UAS GAL4/UAS是存在于酵母中的基因表达调控系统。 UAS是upstream activating sequence的缩写。GAL4是转录调控因子，其Binding domain（BD）与UAS序列结合后，其Activity domain（AD）和promoter区域结合，从而诱导基因的表达。目前，GAL4/UAS系统已广泛应用于各种基因调控研究中。虽然这是一个起源于酵母的...