Gal4–UAS system (Gal4–upstream activating sequence system). A dual system for gene regulation, which is mostly used in flies. The UAS is placed upstream of the gene of interest and is only activated after exp
Similarly, Gal4DBD was fused with ABI1 and VP16 with PYL1. Thus after transfecting this ABA-activator cassette and UAS-driven reporter into mammalian cells, ABA significantly induced the reporter’s production (Figure 3C) [63]. Compared to the rapamycin system, the ABA system has two ...