●高特异性:GAL4只在其启动子允许的细胞类型中表达,并且只与UAS序列结合,从而实现高度特异的基因表达控制。 ●灵活性:可以通过选择不同的启动子和UAS驱动的靶基因来实现各种不同的基因调控实验。 总结 GAL4/UAS系统是一种强大且灵活的基因表达调控工具,广泛应用于基因功能研究和基因调控网络的探索。通过精确控制基因...
becomesthecoreofresearchinpost—genomeeraIn1909,THMorganbeganhisresearchonDrosophilamelanogasterinColumbiaUniversity.Duringtheonecenturyafterthat,thefruitflybecomesallexcellentmodelanimalforgeneticsespeciallythedevelopmentgenetics,andmanybiotechnologiesarise.GALA-UASsystem,whichcamefrom1990’s,isagoodsystemtoexpressexogenous...
UAS-Gal4 Systemdoi:10.1007/3-540-29623-9_9073UAS-Gal4 system refers to an in vivo system that has been adapted from yeast, and consists of two transgenic lines of Drosophila– a driver line (yeast transcription factor Gal4 is expressed under...Springer Berlin Heidelberg...
willcrosstheUASlinetodifferentGALAlineafter theinsertionthe mapping using lines. balancer word:Function Key analysisDrosophilamelanogaster GAlA—UAS Retinitis system Pigmentosa 2 果蝇GAL4一UAS系统分析人类基因功能技术的初步建立 目Ⅱ 舀 长久以来,基因功能分析特别是人类基因功能的分析始终是生物学、遗传 学的...
Synonyms Bipartite GAL4 /UAS expression system Definition The GAL4 /UAS system refers to the use of yeast proteins which interact to induce the expression of genes from any organism in a tissue and temporal-specific manner. Characteristics The GAL4 /UAS System There are many tools available ...
sofarthereisnoefficientsystemtoprovidefunctionalcluesforhumangenesinlargescale.Becauseofitspowerfulgeneticsandrelativelylowcost,DrosophilamelanogasterisaverypopularmodeloverexpressedwithseveralGAL4lines.TheseresultssuggestedthatoverexpressinghumangenesinDrosophilacouldbeusedasaprimaryfunctionalscreen.1MaterialsandMethods1.1Plasmid...
果蝇GAL4/UAS系统在本科遗传学实验中的应用 Application of Drosophila GAL4/UAS System in Undergraduate Genetics Experiment 作者： 马沛勤 苏仙绒 陈莉 作者机构： 运城学院生命科学系,山西运城044000 出版物刊名： 运城学院学报 页码： 67-69页 年卷期： 2014年 第5期 主题词： GAL4-UAS系统 果蝇 遗传实验...
One widely used binary expression system is the GAL4/UAS system, which consists of a driver construct, where expression of the heterologous transactivator GAL4 is driven by an inducible or tissue specific enhancer. In the responder construct, the gene of interest is under the control of the ...
Objective To imitate a genetic screen for visual nervous system genes in drosophila by means of GAL4/UAS system.Methods The ninaE-Gal4/+ and W1118/+ flies ... 杨治平,陈晓姣,李建波,... - 《新疆医科大学学报》 被引量: 0发表: 2018年 应用GAL4/UAS异位表达系统筛选果蝇视觉基因的研究 目的从...
Combining GAL4 /UAS System and CyO-GFP Balancer to Obtain RNA Interference Sequences-expressing Drosophila Larvae in Which Tis11 is Down-regulated Drosophila TIS11 is a homologue of the mammalian post-transcriptional regulation factor TTP(Tristetraproline). TIS11 plays crucial roles in the immunit...