Drosophila in cancer research Introduction of the yeast UAS/GAL4 system into the fly has made such ectopic expression studies easier and more versatile38. With the UAS/GAL4 system, only the construction of a UAS–cDNA construct that drives the gene of interest is required. A fly line carrying...
Definition UAS-Gal4 system refers to an in vivo system that has been adapted from yeast, and consists of two transgenic lines of Drosophila – a driver line (yeast transcription factor Gal4 is expressed under the control of a specific promoter) and a responder line UAS. It is promoter ...
4. The drug-stabilizable Gal4 variants 1xDHFR22 and 2xDHFR22 function in vivo in the format of the widely used Gal4-UAS bipartite expression system for Drosophila. (a) Schematic representation of the constructs used to create drug-inducible Gal4 driver lines. 1xDHFR22 encodes the single DHF...
The Gal4-UAS system has been a go-to tool for researchers working withDrosophila melanogasterfor nearly thirty years. GAL4, a transcription factor originating in yeast, will pair with an upstream activation sequence, or UAS, to manipulate the expression of a gene of interest anywhere in the fl...
In our study we tested the feasibility of annotating the human gene functions in Drosophila with this system by using ten human genes randomly selected. When driven by different GAL4 lines , one of these ten human genes showed potentially conserved signaling pathways. Further investigation of the ...
GAL4一UAS system,we studied the possibility of expressing and functionally characterization of human genes in Drosophila.Fifty-four transgenic fly lines corresponding to 10 human genes have been established.When expressed individually by crossing to an array of 6 different GAL4 driver lines,one of ...
UAS是能增加任何基因转录的模式元件,其相互作用与方向和距离无关,是一种增强子序列。 1.2果蝇GAL4/UAS系统的构建 黑腹果蝇(Drosophilamelanogaster)属于昆虫纲、双翅目、果蝇科、果蝇属,具有许多优良的特性。 收稿日期:2014-08-21 基金项目:山西省教育厅项目(J2013095);运城学院院级项目(JG-201313) ...
willcrosstheUASlinetodifferentGALAlineafter theinsertionthe mapping using lines. balancer word:Function Key analysisDrosophilamelanogaster GAlA—UAS Retinitis system Pigmentosa 2 果蝇GAL4一UAS系统分析人类基因功能技术的初步建立 目Ⅱ 舀 长久以来,基因功能分析特别是人类基因功能的分析始终是生物学、遗传 学的...
Application of Drosophila GAL4/UAS System in Undergraduate Genetics Experiment 作者： 马沛勤 苏仙绒 陈莉 作者机构： 运城学院生命科学系,山西运城044000 出版物刊名： 运城学院学报 页码： 67-69页 年卷期： 2014年 第5期 主题词： GAL4-UAS系统 果蝇 遗传实验 摘要：GAL4蛋白是酵母中的一类转录因子,它...
基金 国家自然科学基金项目(31402021) 关键词 黑腹果蝇 Gal4/UAS系统 Gal80ts UAS转基因 基因表达水平 荧光强度 Drosophila melanogaster GAL4/UAS system Gal80ts UAS transgene gene expression level fluorescence intensity 分类号 Q966 [生物学—昆虫学] 登录...