GAL4/UAS系统是一种广泛应用于果蝇(Drosophila melanogaster)和其他模式生物的基因表达调控系统。它最初是从酵母(Saccharomyces cerevisiae)中引入的,用于研究基因功能和调控。该系统利用了GAL4转录因子和UAS(Upstream Activating Sequence,上游激活序列)之间的特异性结合。 组成部分 1. GAL4基因 GAL4是一种转录因子,能够...
Quantitative analysis of the GAL4/ UAS system in Drosophila oogenesis. Genesis 2006; 44:66-74; PMID:16425298; http://dx.doi.org/10.1002/ gene.20184Goentoro LA,Yakoby N,Goodhouse J,Schupbach T,Shvartsman SY.Quantitative analysis of the GAL4/UAS system in Drosophila oogenesis.Genesis. 2006...
effectively.By using germline transformation together with GAL4一UAS system,we studied the possibility of expressing and functionally characterization of human genes in Drosophila.Fifty-four transgenic fly lines corresponding to 10 human genes have been established.When expressed individually by crossing to...
croppeduas果蝇genesdrosophilacyo OverexpressionofHumanGenesinDrosophilamelanogasterbyUsingGAL42UASSystemMAXi2Zhi,CAILi2Jun,WUXiao2Hui,ZHAOShou2Yuan,LIChang2Ben,DENGKe2Jing3(Morgan2TanInternationalCenterforLifeSciences,InstituteofDevelopmentalBiologyandMolecularMedicine,SchoolofLifeScience,FudanUniversity,Shanghai200433...
Also in Drosophila GAL4-VP16 is more active than GAL4, but it has been shown to be less useful compared to GAL4Δ given that many insertions of GAL4-VP16 constructs appear to be non-functional [23]. After its inception in the mouse [29], the Gal4/UAS system has been established ...
willcrosstheUASlinetodifferentGALAlineafter theinsertionthe mapping using lines. balancer word:Function Key analysisDrosophilamelanogaster GAlA—UAS Retinitis system Pigmentosa 2 果蝇GAL4一UAS系统分析人类基因功能技术的初步建立 目Ⅱ 舀 长久以来,基因功能分析特别是人类基因功能的分析始终是生物学、遗传 学的...
Characteristics The GAL4 /UAS System There are many tools available when using the fruitfly, Drosophila melanogaster , a model organism highly amenable to genetic analysis. One particular tool is the GAL4 /UAS system that allows for the expression of genes from any organism in a tissue and ...
关键词:功能分析黑腹果蝇GAL4一UAS系统视网膜色素变性果蝇GAIA-UAS系统分析人类基因功能技术的初步建立AbstraclTheHumanGenomeprojectwillbecompletedlately.Thentheanalysisofgenefunctionbecomesthecoreofresearchinpost—genomeeraIn1909,THMorganbeganhisresearchonDrosophilamelanogasterinColumbiaUniversity.Duringtheonecenturyafterthat,...
Application of Drosophila GAL4/UAS System in Undergraduate Genetics Experiment 作者： 马沛勤 苏仙绒 陈莉 作者机构： 运城学院生命科学系,山西运城044000 出版物刊名： 运城学院学报 页码： 67-69页 年卷期： 2014年 第5期 主题词： GAL4-UAS系统 果蝇 遗传实验 摘要：GAL4蛋白是酵母中的一类转录因子,它...
Enhancer trapping would be a useful but primarily descriptive technique, if it were not for the development of the GAL4/UAS system (Brand and Perrimon, 1993). Here, the reporter gene is the yeast transcription factor GAL4. This recognises no sites in the natural Drosophila genome, and s...