Wenwen Lv,Jie Ding,Jiangbo Hu,Chao Wang,Leyi Xu,Ming Fang,Liudi Yuan. A GAL4/UAS luciferase system to identify the miRNAs target mRNAs in Drosophila S2 cells[J]. Molecular Biology Reports . 2009 (6)Wenwen Lv,Jie Ding,Jiangbo Hu,Chao Wang,Leyi Xu,Ming Fang,Liudi Yuan.A GAL4/...
GAL4-Luc萤光素酶报告基因(报告基因质粒)(GAL4 luciferase reporter plasmid)是翌圣生物自主研发的用于检测GAL4转录活性水平为目的的报告基因。GAL4是酵母(Saccharomyces cerevisiae)转录激活蛋白Gal4的基因,是酵母半乳糖的代谢相关基因,GAL4/UAS系统被用于基因表达研究的一个有效工具。
在半乳糖的诱导下,酵母Gal4结合在半乳糖代谢酶系GAL基因启动子的上游激活序列(Upstream activating sequence,UAS)上,调控Galactokinase(Gal 1),Galactose permease(Gal2),Galactose 1-phosphate uridyltransferase(Gal7),Galactose mutarotase and UDP-galactose 4-epimerase(Gal10)等基因的转录。 酵母单杂交与酵母双杂交...
一UAS and Dual —L^lc】 涨reporter gene system. R esults:Lucifc“ I se activitie s detected by t h e Gal4/ VP 16 一UAS and Dual —Luciferase reporter gcne assay s ystem were increased by GMI , in paralld wit h the increase of AB secretiondetected by irranu noprecipitaf i on ...
GAL4UAS-Luciferase reporter质粒 货号:#64125-EA 货号产品名称规格库存价格数量购买 #64125-EA GAL4UAS-Luciferase reporter质粒 EA 立即咨询 + - 产品介绍 相关产品 》产品信息 货号 名称 规格 别名 #64125 GAL4UAS-Luciferase reporter质粒 EA 》产品描述 哺乳细胞转录因子报告Promoter 》产品简介 Plasmid...
在半乳糖的诱导下,酵母Gal4结合在半乳糖代谢酶系GAL基因启动子的上游激活序列(Upstream activating sequence,UAS)上,调控Galactokinase(Gal 1),Galactose permease(Gal2),Galactose 1-phosphate uridyltransferase(Gal7),Galactose mutarotase and UDP-galactose 4-epimerase(Gal10)等基因的转录。
The development of the bipartite Gal4-UAS system in Anopheles gambiae would improve the functional characterization of genes in this important malaria vector. Towards this aim, we used Gal4 driver plasmids to successfully activate expression of the reporter gene, luciferase, from UAS responder ...
The predicted miR-275 target gene- sarco/endoplasmic reticulum Ca2+-ATPase (SERCA)-was confirmed to directly bind miR-275 by means of luciferase assay. RNA interference silencing of SERCA or application of SERCA-specific inhibitor Thapsigargin (TG) resulted in an apparent lack of blood digestion,...