Quantitative analysis of the GAL4/UAS system in Drosophila oogenesis. Genesis 44, 66-74.Goentoro LA,Yakoby N,Goodhouse J,Schupbach T,Shvartsman SY.Quantitative analysis of the GAL4/UAS system in Drosophila ooge
We show the functioning of the new drug-inducible Gal4 system in the format of the widely used Gal4-UAS bipartite expression system in vivo. Finally, we demonstrate the tightness of the regulation provided by the least background architecture, by regulating the expression of a highly toxic ...
Drosophila in cancer research Introduction of the yeast UAS/GAL4 system into the fly has made such ectopic expression studies easier and more versatile38. With the UAS/GAL4 system, only the construction of a UAS–cDNA construct that drives the gene of interest is required. A fly line carrying...
The Gal4-UAS system has been a go-to tool for researchers working withDrosophila melanogasterfor nearly thirty years. GAL4, a transcription factor originating in yeast, will pair with an upstream activation sequence, or UAS, to manipulate the expression of a gene of interest anywhere in the fl...
effectively.By using germline transformation together with GAL4一UAS system,we studied the possibility of expressing and functionally characterization of human genes in Drosophila.Fifty-four transgenic fly lines corresponding to 10 human genes have been established.When expressed individually by crossing to...
In our study we tested the feasibility of annotating the human gene functions in Drosophila with this system by using ten human genes randomly selected. When driven by different GAL4 lines , one of these ten human genes showed potentially conserved signaling pathways. Further investigation of the ...
1.2果蝇GAL4/UAS系统的构建 黑腹果蝇(Drosophilamelanogaster)属于昆虫纲、双翅目、果蝇科、果蝇属,具有许多优良的特性。 收稿日期:2014-08-21 基金项目:山西省教育厅项目(J2013095);运城学院院级项目(JG-201313) ( 67 )作者简介:马沛勤(1960-)女,山西运城人,运城学院生命科学系副教授,研究方向为遗传进化。
Application of Drosophila GAL4/UAS System in Undergraduate Genetics Experiment 作者： 马沛勤 苏仙绒 陈莉 作者机构： 运城学院生命科学系,山西运城044000 出版物刊名： 运城学院学报 页码： 67-69页 年卷期： 2014年 第5期 主题词： GAL4-UAS系统 果蝇 遗传实验 摘要：GAL4蛋白是酵母中的一类转录因子,它...
of4adults genetic eye willcrosstheUASlinetodifferentGALAlineafter theinsertionthe mapping using lines. balancer word:Function Key analysisDrosophilamelanogaster GAlA—UAS Retinitis system Pigmentosa 2 果蝇GAL4一UAS系统分析人类基因功能技术的初步建立 目Ⅱ 舀 长久以来,基因功能分析特别是人类基因功能的分析始终...
摘要 【目的】Gal80ts与Gal4组合驱动UAS转基因表达是黑腹果蝇Drosophila melanogaster研究中常用的转基因过表达遗传学工具,通过温度控制实现对UAS转基因表达的灵活开关。Ga...展开更多 【Aim】The combination of Gal80^(ts)and Gal4 is commonly used to regulate the UAS transgene overexpression in Drosophila,and...