Synonyms Bipartite GAL4 /UAS expression system Definition The GAL4 /UAS system refers to the use of yeast proteins which interact to induce the expression of genes from any organism in a tissue and temporal-specific manner. Characteristics The GAL4 /UAS System There are many tools available ...
Introduction of the yeast UAS/GAL4 system into the fly has made such ectopic expression studies easier and more versatile38. With the UAS/GAL4 system, only the construction of a UAS–cDNA construct that drives the gene of interest is required. A fly line carrying the UAS–cDNA construct can...
line, the progeny expresses the GAL4 protein in a pattern specified by the promoter used to make the GAL4 construct, which binds to the UAS sequence, driving expression of the downstream construct (Fig. 4.1). The GAL4/UAS system has become theworkhorse in Drosophila research, and many ...
The Gal4-UAS system has been a go-to tool for researchers working withDrosophila melanogasterfor nearly thirty years. GAL4, a transcription factor originating in yeast, will pair with an upstream activation sequence, or UAS, to manipulate the expression of a gene of interest anywhere in the fl...
洗膜液1:Urea6M,O.4%SDS,0.5×SSC 洗膜液2:2×SSC (4) 限制性内切酶: Mannheim公司 果蝇GAL4.UAS系统分析人类基因功能技术的初步建立 DNATaq、PCRbuf、Mgcl2 dNTP等购自Promega公司 T4 DNA连接酶、RnaseA购ft Mannheim公司 Boringer 主要仪器与设备’ ELMER公司1 PCR仪:PCR扩增仪GeneAmpPCRsystem2400(美国...
何通过GAL4-UAS系统在模式动物黑腹果蝇中实现靶基因的时空表达,认识GAL4-UAS系统在研究果蝇遗传发育中的重要性和优越性,理解分子调控、靶基因表达与个体的遗传和发育每个环节都是可以操作和控制的现代遗传学实验理念。 关键词:GAL4-UAS系统;果蝇;遗传实验
GAL4一UAS system,we studied the possibility of expressing and functionally characterization of human genes in Drosophila.Fifty-four transgenic fly lines corresponding to 10 human genes have been established.When expressed individually by crossing to an array of 6 different GAL4 driver lines,one of ...
arise.GALA-UAS system,which camefrom 1990’s,is a goodsystem to expressexogenousgene and analyze itsfunction Manypapers were published using this system. WehaveclonedthecDNAofRetinitis Pigmentosa 2and epitopec-myc into pUAST vectorand inducedits expression inSchneidercellline2 disaggregated from late ...
基金 国家自然科学基金项目(31402021) 关键词 黑腹果蝇 Gal4/UAS系统 Gal80ts UAS转基因 基因表达水平 荧光强度 Drosophila melanogaster GAL4/UAS system Gal80ts UAS transgene gene expression level fluorescence intensity 分类号 Q966 [生物学—昆虫学] 登录...
内容提示: 代生 医 进展Pro lw~ in Modern Biomedidne 2006 Vo 1. 6 No. 3 · 1 - ·基础研究· 利用Gal4/ VP16一 UAS和双荧光素酶报告基因 系统检测,),一 分泌酶活性 刘忠华 (北京大学精神卫生研究所 北京 100083) 阮燕 查芹芹 张岱 摘要目的: 确立基于Ga l4/ vpl6一uAS 和双荧光素酶报告...